Transcript: Human XM_017003982.1

PREDICTED: Homo sapiens cyclin dependent kinase like 4 (CDKL4), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDKL4 (344387)
Length:
2847
CDS:
245..1273

Additional Resources:

NCBI RefSeq record:
XM_017003982.1
NBCI Gene record:
CDKL4 (344387)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149006 CCGATTATGTAGCTACGAGA pXPR_003 TGG 489 48% 6 0.6774 CDKL4 CDKL4 76107
2 BRDN0001146173 AAATCTTGTGAACCTCATCG pXPR_003 AGG 199 19% 3 0.6364 CDKL4 CDKL4 76106
3 BRDN0001147188 GTGGCCTGGAAAATCAGATG pXPR_003 TGG 616 60% 6 0.532 CDKL4 CDKL4 76109
4 BRDN0001149476 AATATTCTAATAACTAAGCA pXPR_003 AGG 407 40% 5 -0.2820 CDKL4 CDKL4 76108
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003982.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358265 ATGGTTCTTCAGTCGATATAT pLKO_005 777 CDS 100% 15.000 21.000 N CDKL4 n/a
2 TRCN0000021521 CCGATTATGTAGCTACGAGAT pLKO.1 717 CDS 100% 4.050 5.670 N CDKL4 n/a
3 TRCN0000021523 GTATGGTTCTTCAGTCGATAT pLKO.1 775 CDS 100% 10.800 8.640 N CDKL4 n/a
4 TRCN0000358262 ACGTATGTTGAAGCAATTAAA pLKO_005 400 CDS 100% 15.000 10.500 N CDKL4 n/a
5 TRCN0000358263 GAGATGCCTACACCGATTATG pLKO_005 705 CDS 100% 13.200 9.240 N CDKL4 n/a
6 TRCN0000021520 CCAAGACATCAATCAATCTTT pLKO.1 908 CDS 100% 5.625 3.938 N CDKL4 n/a
7 TRCN0000021522 CTGAAGATGATCCTGTTGTTA pLKO.1 357 CDS 100% 5.625 3.938 N CDKL4 n/a
8 TRCN0000196305 GAGCTCCTACTTTGATTCTTT pLKO.1 1090 CDS 100% 5.625 3.938 N CDKL4 n/a
9 TRCN0000195080 CCAAATCTTGTGAACCTCATC pLKO.1 425 CDS 100% 4.050 2.835 N CDKL4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003982.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000467405 GCGTACAAATGTAGATCTTTTTCC pLX_317 30.2% 90.8% 19.5% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_15308 pDONR223 100% 90.5% 19.5% None (many diffs) n/a
3 ccsbBroad304_15308 pLX_304 0% 90.5% 19.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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