Construct: ORF TRCN0000467405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018818.2_s317c1
- Derived from:
- ccsbBroadEn_15308
- DNA Barcode:
- GCGTACAAATGTAGATCTTTTTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKL4 (344387)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | NM_001009565.2 | 99.3% | 21.2% | (many diffs) |
| 2 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_011532816.2 | 90.8% | 19.5% | (many diffs) |
| 3 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_017003981.2 | 90.8% | 19.5% | (many diffs) |
| 4 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_017003982.1 | 90.8% | 19.5% | (many diffs) |
| 5 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | NM_001346911.1 | 82.4% | 17.6% | (many diffs) |
| 6 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_011532815.3 | 82.4% | 17.6% | (many diffs) |
| 7 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_011532817.2 | 78.8% | 22.1% | (many diffs) |
| 8 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_011532819.2 | 57.7% | 33.6% | (many diffs) |
| 9 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | NR_144520.1 | 54.3% | (many diffs) | |
| 10 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_017003984.1 | 53.3% | 35.6% | (many diffs) |
| 11 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XM_017003985.1 | 53.2% | 38% | (many diffs) |
| 12 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | NR_144521.1 | 50.1% | (many diffs) | |
| 13 | human | 344387 | CDKL4 | cyclin dependent kinase like 4 | XR_001738736.1 | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 306
- ORF length:
- 237
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaagtat gaaaaattag ctaagactgg agaagggtct tatggggttg 121 tattcaaatg cagaaacaaa acctctggac aagtagtagc tgttaaaaaa tttgtggaat 181 ctgaagatga tcctgttgtt aagaaaatag cactaagaga aatacgtatg ttgaagcaaa 241 ttaaaacaat nccaaatctt gtgaacctca tcgaaggtgt tcaggagaaa aaaggaaaat 301 gcatttagtt tttgaatact gtgatcatac acttttaaat gagctggaaa gaaacccaaa 361 tggagttgct gatggagtga tcaaaagcgt attatggcaa acacttcaag ctcttaattt 421 ctgtcatata cataactgta ttcacagaga tataaaacct gaaaatattc taataactaa 481 gcaaggaata atcaagattt gtgacttcgg gtttgcacaa attctgattc caggagatgc 541 ctacaccgat tatgtagcta cgagatggta ccgagctcct gaacttcttg tgggagatac 601 tcagtatggt tcttcagtcg atatatgggc tattggttgt gtttttgcag agctcctgac 661 aggccagcca ctgtggcctg gaaaatcaga tgtggaccaa ctttatctga taatcagaac 721 actaggaaaa ttaatCCCAA GACATCAATC AATCTTTAAA AGTAACGGGT TTTTCCATGG 781 CATCAGTATA CCTGAGCCAG AAGACATGGA AACTCTTGAG GAAAAGTTCT CAGATGTTCA 841 TCCTGTGGCT CTGAACTTCA TGAAGGGGTG TCTGAAGATG AATCCAGATG ACAGATTAAC 901 CTGTTCCCAA CTCCTGGAGA GCTCCTACTT TGATTCTTTT CAAGAGGCCC AAATTAAAAG 961 AAAAGCACGT AATGAAGGAA GAAACAGAAG ACGCCAACAG GTACTTCCGC TCAAAAGTTT 1021 GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 1081 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 1141 TTATATATCT TGTGGAAAGG ACGAGCGTAC AAATGTAGAT CTTTTTCCAC GCGTTAAGTC 1201 gacaatcaac ctctggatta caaaatttgt gaaagatt