Transcript: Human XM_017007993.1

PREDICTED: Homo sapiens cyclin G associated kinase (GAK), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GAK (2580)
Length:
4357
CDS:
301..3960

Additional Resources:

NCBI RefSeq record:
XM_017007993.1
NBCI Gene record:
GAK (2580)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146263 ACTTGTTGCTTAGTAACCAA pXPR_003 GGG 141 4% 4 0.4087 GAK GAK 76471
2 BRDN0001146687 CCCAGCATCATACTGAGCCG pXPR_003 TGG 1876 51% 18 -0.1490 GAK GAK 76470
3 BRDN0001148048 TGTACTGCGTGTCGTGCGGG pXPR_003 GGG 431 12% 6 -0.3358 GAK GAK 76472
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017007993.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199033 CCGAAGGAACAGCTGATTCAT pLKO.1 4165 3UTR 100% 5.625 7.875 N GAK n/a
2 TRCN0000352695 CCGAAGGAACAGCTGATTCAT pLKO_005 4165 3UTR 100% 5.625 7.875 N GAK n/a
3 TRCN0000196463 GAAGATCTGTTGTCCAATCAA pLKO.1 3565 CDS 100% 5.625 7.875 N GAK n/a
4 TRCN0000342526 GAAGATCTGTTGTCCAATCAA pLKO_005 3565 CDS 100% 5.625 7.875 N GAK n/a
5 TRCN0000204887 CCGTCGCTAATTATGCAAAGG pLKO.1 1088 CDS 100% 4.050 5.670 N GAK n/a
6 TRCN0000195055 CCAGAAATCATAGACTTGTAT pLKO.1 577 CDS 100% 5.625 4.500 N GAK n/a
7 TRCN0000196794 GAGAACTTGTTGCTTAGTAAC pLKO.1 421 CDS 100% 10.800 7.560 N GAK n/a
8 TRCN0000002158 AGCATTCCAAAGCCTCTGATT pLKO.1 4124 3UTR 100% 4.950 3.465 N GAK n/a
9 TRCN0000002154 CCAGAATTGCAGTGATGTCAT pLKO.1 1136 CDS 100% 4.950 3.465 N GAK n/a
10 TRCN0000002157 CCCGGATTTATTTCAAGTGAA pLKO.1 1983 CDS 100% 4.950 3.465 N GAK n/a
11 TRCN0000342470 CCCGGATTTATTTCAAGTGAA pLKO_005 1983 CDS 100% 4.950 3.465 N GAK n/a
12 TRCN0000195054 CCTCTGATTGTTGTTTCCTTT pLKO.1 4136 3UTR 100% 4.950 3.465 N GAK n/a
13 TRCN0000002156 GAAGATGGCAAAGCGGTGATT pLKO.1 1762 CDS 100% 4.950 3.465 N GAK n/a
14 TRCN0000342525 GAAGATGGCAAAGCGGTGATT pLKO_005 1762 CDS 100% 4.950 3.465 N GAK n/a
15 TRCN0000002155 GCCTAACTATGCCTCGAACTT pLKO.1 3462 CDS 100% 4.950 3.465 N GAK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017007993.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.