Transcript: Human XM_017008581.2

PREDICTED: Homo sapiens TXK tyrosine kinase (TXK), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TXK (7294)
Length:
6814
CDS:
1173..2681

Additional Resources:

NCBI RefSeq record:
XM_017008581.2
NBCI Gene record:
TXK (7294)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145857 AGAACATCTATTGAGACAAG pXPR_003 AGG 421 28% 5 0.6442 TXK TXK 76358
2 BRDN0001146212 TGGTCCATTTAGGTGAATGG pXPR_003 CGG 792 52% 9 0.2541 TXK TXK 76360
3 BRDN0001145866 GGAAGGCAAGAGACCGTTTG pXPR_003 GGG 300 20% 3 -0.2161 TXK TXK 76359
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017008581.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121108 CCTACACAATTTCCGTATTTA pLKO.1 1645 CDS 100% 15.000 21.000 N TXK n/a
2 TRCN0000121181 GAGTCTAAAGAAGGTGCATTT pLKO.1 1596 CDS 100% 10.800 15.120 N TXK n/a
3 TRCN0000121180 GCACCAATGTCCATATATGAA pLKO.1 2568 CDS 100% 5.625 7.875 N TXK n/a
4 TRCN0000195084 CAAGGAATTGTTTGGTCAGTT pLKO.1 2275 CDS 100% 4.950 6.930 N TXK n/a
5 TRCN0000009998 CCGTATTTATGGGAGCTAGAA pLKO.1 1657 CDS 100% 4.950 6.930 N TXK n/a
6 TRCN0000055429 CCGTATTTATGGGAGCTAGAA pLKO.1 1657 CDS 100% 4.950 6.930 N TXK n/a
7 TRCN0000121111 CCTTTACATTGTGACAGAGTT pLKO.1 2111 CDS 100% 4.950 6.930 N TXK n/a
8 TRCN0000009999 GACACGCCTTTCAATCAATCC pLKO.1 1750 CDS 100% 4.050 5.670 N TXK n/a
9 TRCN0000196957 GCAAGTCGTGGAAGCTATTTC pLKO.1 2516 CDS 100% 13.200 10.560 N TXK n/a
10 TRCN0000121110 GCCCAGAGAACCCTGTAATTT pLKO.1 1379 CDS 100% 15.000 10.500 N TXK n/a
11 TRCN0000196460 GCTCAGCCAATTGTCAAATAA pLKO.1 1244 CDS 100% 15.000 10.500 N TXK n/a
12 TRCN0000194951 CAGTGATCCATGCATAGAATA pLKO.1 3061 3UTR 100% 13.200 9.240 N TXK n/a
13 TRCN0000194938 CAGTTCAACATGCATAGTAAA pLKO.1 2291 CDS 100% 13.200 9.240 N TXK n/a
14 TRCN0000314836 CAGTTCAACATGCATAGTAAA pLKO_005 2291 CDS 100% 13.200 9.240 N TXK n/a
15 TRCN0000121107 CCCTTGAAGAATTTCATGTTT pLKO.1 3121 3UTR 100% 5.625 3.938 N TXK n/a
16 TRCN0000194674 CCTGAGTTAATCTGGTATCAC pLKO.1 1770 CDS 100% 4.950 3.465 N TXK n/a
17 TRCN0000196365 GCACCTTTATAAATCACATGA pLKO.1 3170 3UTR 100% 4.950 3.465 N TXK n/a
18 TRCN0000314838 GCACCTTTATAAATCACATGA pLKO_005 3170 3UTR 100% 4.950 3.465 N TXK n/a
19 TRCN0000121177 GCCAACCCAAAGAGTCATCTT pLKO.1 2695 3UTR 100% 4.950 3.465 N TXK n/a
20 TRCN0000314784 GCCAACCCAAAGAGTCATCTT pLKO_005 2695 3UTR 100% 4.950 3.465 N TXK n/a
21 TRCN0000121288 GTCCATATATGAAGTCATGTA pLKO.1 2576 CDS 100% 4.950 3.465 N TXK n/a
22 TRCN0000001577 GAAACAGAATGCCAACCCAAA pLKO.1 2685 3UTR 100% 4.050 2.835 N TXK n/a
23 TRCN0000314837 GAAACAGAATGCCAACCCAAA pLKO_005 2685 3UTR 100% 4.050 2.835 N TXK n/a
24 TRCN0000121109 GCTTAACTATCTCAGGGAGAA pLKO.1 2150 CDS 100% 4.050 2.835 N TXK n/a
25 TRCN0000121291 TGCTGGCATGAGAAACCTGAA pLKO.1 2601 CDS 100% 4.050 2.835 N TXK n/a
26 TRCN0000001580 GCTGCCTGCTTAACTATCTCA pLKO.1 2143 CDS 100% 3.000 2.100 N TXK n/a
27 TRCN0000314783 GCTGCCTGCTTAACTATCTCA pLKO_005 2143 CDS 100% 3.000 2.100 N TXK n/a
28 TRCN0000121290 GTCAAATAAGAAGCAATCCAA pLKO.1 1256 CDS 100% 3.000 2.100 N TXK n/a
29 TRCN0000121179 GCTGTCACAGAGATTGCGGAA pLKO.1 2652 CDS 100% 2.160 1.512 N TXK n/a
30 TRCN0000009997 TACCTGATACTGGAGAAATAC pLKO.1 1422 CDS 100% 0.000 0.000 N TXK n/a
31 TRCN0000121178 CCAGGATATATGTGAAGGAAT pLKO.1 2210 CDS 100% 4.950 2.970 N TXK n/a
32 TRCN0000121287 CCCAAATGTGAAGAAGTGAAA pLKO.1 2919 3UTR 100% 4.950 2.970 N TXK n/a
33 TRCN0000001579 GAATACCTGATACTGGAGAAA pLKO.1 1419 CDS 100% 4.950 2.970 N TXK n/a
34 TRCN0000196461 GAAGAGAAGATCCAAGTCAAG pLKO.1 1341 CDS 100% 4.050 2.430 N TXK n/a
35 TRCN0000121289 GAGTGGTACCATAGAAACATT pLKO.1 1542 CDS 100% 0.000 0.000 N TXK n/a
36 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 5037 3UTR 100% 4.950 2.475 Y ORAI2 n/a
37 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 5126 3UTR 100% 10.800 5.400 Y SMIM11A n/a
38 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 5034 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017008581.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14869 pDONR223 0% 95.2% 95.2% None 0_1ins75 n/a
2 ccsbBroad304_14869 pLX_304 0% 95.2% 95.2% V5 0_1ins75 n/a
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