Transcript: Human XM_017011904.1

PREDICTED: Homo sapiens adenosylhomocysteinase like 2 (AHCYL2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AHCYL2 (23382)
Length:
4783
CDS:
403..1617

Additional Resources:

NCBI RefSeq record:
XM_017011904.1
NBCI Gene record:
AHCYL2 (23382)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017011904.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050167 GCAGAGTTTGGACGAAGAGAA pLKO.1 361 5UTR 100% 4.950 6.930 N AHCYL2 n/a
2 TRCN0000050165 CCGTATGAAGAATAGCTGCAT pLKO.1 1182 CDS 100% 2.640 3.696 N AHCYL2 n/a
3 TRCN0000050163 CCAAACATGATCTTGGATGAT pLKO.1 691 CDS 100% 4.950 3.465 N AHCYL2 n/a
4 TRCN0000050166 GCTCTAGCAGAAAGTGGATTT pLKO.1 589 CDS 100% 10.800 6.480 N AHCYL2 n/a
5 TRCN0000050164 GCCTTGATAGAGCTTTACAAT pLKO.1 1417 CDS 100% 5.625 3.375 N AHCYL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017011904.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02757 pDONR223 100% 66.1% 66.1% None 0_1ins621 n/a
2 ccsbBroad304_02757 pLX_304 0% 66.1% 66.1% V5 0_1ins621 n/a
3 TRCN0000481020 AAAGACACGACGTGGTCGTCTCCA pLX_317 22% 66.1% 66.1% V5 0_1ins621 n/a
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