Construct: ORF ccsbBroadEn_02757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004016.1_s300c1, BRDN0000456648
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHCYL2 (23382)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_015328.4 | 100% | 100% | |
| 2 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130720.3 | 99.8% | 99.8% | 361_362insAGC |
| 3 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130723.3 | 81.7% | 80.8% | (many diffs) |
| 4 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_005250237.1 | 81.7% | 81% | (many diffs) |
| 5 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_011515987.2 | 81.7% | 81% | (many diffs) |
| 6 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_011515988.2 | 81.7% | 81% | (many diffs) |
| 7 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130722.3 | 81% | 80.6% | (many diffs) |
| 8 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011904.1 | 66.1% | 66.1% | 0_1ins621 |
| 9 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011905.2 | 66.1% | 66.1% | 0_1ins621 |
| 10 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011906.1 | 66.1% | 66.1% | 0_1ins621 |
| 11 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011907.1 | 66.1% | 66.1% | 0_1ins621 |
| 12 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242675.2 | 62.5% | 73.3% | (many diffs) |
| 13 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242676.2 | 62.5% | 73.3% | (many diffs) |
| 14 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | NM_021414.6 | 92.1% | 98.8% | (many diffs) |
| 15 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | NM_001171000.2 | 91.9% | 98.6% | (many diffs) |
| 16 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | NM_001347084.1 | 75.9% | 80.8% | (many diffs) |
| 17 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_006505182.2 | 75.9% | 81% | (many diffs) |
| 18 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_006505181.3 | 75.6% | 78.3% | (many diffs) |
| 19 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_006505179.2 | 75.5% | 79.1% | (many diffs) |
| 20 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | NM_001171001.1 | 75.5% | 80.6% | (many diffs) |
| 21 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_006505183.3 | 75.4% | 80.5% | (many diffs) |
| 22 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_006505180.2 | 75.3% | 80.3% | (many diffs) |
| 23 | mouse | 74340 | Ahcyl2 | S-adenosylhomocysteine hydr... | XM_011241082.2 | 70.8% | 74.3% | (many diffs) |
| 24 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_006501408.3 | 63.5% | 73.3% | (many diffs) |
| 25 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319556.1 | 41.7% | 48.6% | (many diffs) |
| 26 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319557.1 | 41.7% | 48.6% | (many diffs) |
| 27 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319558.1 | 38.3% | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1899
- ORF length:
- 1833
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC GGTGCAGGTT GTGTCAGCCG CGGCTGCCGC CAAGGTGCCT GAGGTGGAGC 121 TGAAGGACCT GAGCCCCTCC GAGGCGGAGT CGCAACTAGG ACTGAGCACG GCCGCCGTGG 181 GCGCCATGGC CCCCCCGGCG GGCGGTGGAG ACCCTGAGGC TCCAGCTCCC GCCGCGGAGC 241 GGCCCCCGGT CCCCGGCCCG GGCTCGGGGC CCGCCGCCGC TCTCAGCCCC GCCGCCGGGA 301 AGGTGCCTCA GGCGTCGGCC ATGAAGCGGA GCGACCCACA TCACCAGCAC CAGCGGCACC 361 GCGACGGCGG CGAGGCCCTG GTCAGCCCCG ACGGCACCGT CACCGAGGCG CCGCGCACAG 421 TCAAGAAGCA GATCCAGTTT GCTGACCAGA AGCAAGAATT CAACAAACGT CCCACCAAAA 481 TTGGACGTCG CTCTTTGTCT CGTTCCATTT CTCAGTCATC TACTGACAGC TACAGCTCAG 541 CGGCTTCATA TACAGATAGC TCTGATGATG AGACATCGCC CAGGGACAAG CAGCAAAAGA 601 ACTCTAAGGG AAGCAGTGAC TTCTGTGTTA AGAACATCAA ACAGGCAGAG TTTGGACGAA 661 GAGAAATTGA AATTGCTGAA CAAGAAATGC CTGCATTGAT GGCTTTGAGG AAGAGAGCTC 721 AAGGAGAAAA GCCTTTGGCT GGAGCCAAAA TCGTGGGTTG CACACACATC ACTGCTCAGA 781 CTGCTGTGCT TATGGAAACT CTGGGTGCTC TGGGGGCCCA GTGCCGATGG GCTGCCTGCA 841 ACATCTATTC CACTCTCAAT GAAGTGGCTG CTGCTCTAGC AGAAAGTGGA TTTCCTGTTT 901 TTGCCTGGAA GGGAGAGTCA GAAGATGACT TTTGGTGGTG TATCGATAGA TGTGTGAATG 961 TGGAGGGCTG GCAGCCAAAC ATGATCTTGG ATGATGGAGG GGATCTTACC CACTGGATTT 1021 ATAAAAAGTA TCCCAACATG TTTAAGAAAA TCAAGGGCAT AGTAGAGGAG AGTGTTACTG 1081 GAGTTCACAG GCTGTACCAA CTGTCCAAAG CTGGGAAGCT GTGTGTTCCA GCCATGAATG 1141 TCAATGACTC AGTCACCAAA CAGAAATTTG ACAACCTCTA CTGTTGCCGT GAATCAATTC 1201 TTGATGGACT TAAAAGGACA ACAGACATGA TGTTTGGTGG AAAGCAAGTG GTAGTCTGTG 1261 GCTATGGAGA GGTGGGGAAA GGGTGCTGTG CTGCCCTGAA AGCCATGGGC TCCATTGTGT 1321 ATGTAACTGA AATTGACCCC ATCTGTGCCC TGCAAGCCTG TATGGATGGA TTTCGACTGG 1381 TGAAATTAAA TGAGGTCATC CGACAAGTGG ACATTGTTAT TACCTGTACA GGTAACAAGA 1441 ATGTGGTAAC CAGAGAGCAC TTGGACCGTA TGAAGAATAG CTGCATCGTT TGTAACATGG 1501 GACATTCCAA CACAGAGATT GACGTGGCGA GTCTGCGGAC ACCAGAACTG ACCTGGGAGC 1561 GAGTGAGATC TCAAGTTGAC CATGTGATAT GGCCTGATGG CAAGAGGATA GTACTGCTGG 1621 CAGAGGGCCG CCTGCTGAAC CTTAGCTGCT CCACAGTGCC TACATTTGTG CTCTCAATCA 1681 CTGCTACTAC TCAGGCTCTT GCCTTGATAG AGCTTTACAA TGCTCCTGAG GGTCGCTATA 1741 AGCAGGATGT CTACCTGTTG CCCAAGAAGA TGGATGAGTA TGTGGCCAGC CTACACCTGC 1801 CTACCTTTGA TGCCCACTTG ACAGAGCTGA CAGATGAACA GGCCAAGTAT CTGGGACTCA 1861 ACAAGAATGG GCCCTTCAAG CCTAATTACT ACAGGTATTA CCCAACTTNC TTGTACAAAG 1921 TTGGcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac