Construct: ORF ccsbBroad304_02757

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004016.1_s304c1, BRDN0000461607
Derived from:
ccsbBroadEn_02757
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AHCYL2 (23382)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02757
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_015328.4 100% 100%
2 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130720.3 99.8% 99.8% 361_362insAGC
3 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130723.3 81.7% 80.8% (many diffs)
4 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_005250237.1 81.7% 81% (many diffs)
5 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_011515987.2 81.7% 81% (many diffs)
6 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_011515988.2 81.7% 81% (many diffs)
7 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130722.3 81% 80.6% (many diffs)
8 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011904.1 66.1% 66.1% 0_1ins621
9 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011905.2 66.1% 66.1% 0_1ins621
10 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011906.1 66.1% 66.1% 0_1ins621
11 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011907.1 66.1% 66.1% 0_1ins621
12 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242675.2 62.5% 73.3% (many diffs)
13 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242676.2 62.5% 73.3% (many diffs)
14 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... NM_021414.6 92.1% 98.8% (many diffs)
15 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... NM_001171000.2 91.9% 98.6% (many diffs)
16 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... NM_001347084.1 75.9% 80.8% (many diffs)
17 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_006505182.2 75.9% 81% (many diffs)
18 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_006505181.3 75.6% 78.3% (many diffs)
19 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_006505179.2 75.5% 79.1% (many diffs)
20 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... NM_001171001.1 75.5% 80.6% (many diffs)
21 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_006505183.3 75.4% 80.5% (many diffs)
22 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_006505180.2 75.3% 80.3% (many diffs)
23 mouse 74340 Ahcyl2 S-adenosylhomocysteine hydr... XM_011241082.2 70.8% 74.3% (many diffs)
24 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_006501408.3 63.5% 73.3% (many diffs)
25 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319556.1 41.7% 48.6% (many diffs)
26 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319557.1 41.7% 48.6% (many diffs)
27 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319558.1 38.3% 42.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1899
ORF length:
1833
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ggtgcaggtt gtgtcagccg cggctgccgc caaggtgcct gaggtggagc
121tgaaggacct gagcccctcc gaggcggagt cgcaactagg actgagcacg gccgccgtgg
181gcgccatggc ccccccggcg ggcggtggag accctgaggc tccagctccc gccgcggagc
241ggcccccggt ccccggcccg ggctcggggc ccgccgccgc tctcagcccc gccgccggga
301aggtgcctca ggcgtcggcc atgaagcgga gcgacccaca tcaccagcac cagcggcacc
361gcgacggcgg cgaggccctg gtcagccccg acggcaccgt caccgaggcg ccgcgcacag
421tcaagaagca gatccagttt gctgaccaga agcaagaatt caacaaacgt cccaccaaaa
481ttggacgtcg ctctttgtct cgttccattt ctcagtcatc tactgacagc tacagctcag
541cggcttcata tacagatagc tctgatgatg agacatcgcc cagggacaag cagcaaaaga
601actctaaggg aagcagtgac ttctgtgtta agaacatcaa acaggcagag tttggacgaa
661gagaaattga aattgctgaa caagaaatgc ctgcattgat ggctttgagg aagagagctc
721aaggagaaaa gcctttggct ggagccaaaa tcgtgggttg cacacacatc actgctcaga
781ctgctgtgct tatggaaact ctgggtgctc tgggggccca gtgccgatgg gctgcctgca
841acatctattc cactctcaat gaagtggctg ctgctctagc agaaagtgga tttcctgttt
901ttgcctggaa gggagagtca gaagatgact tttggtggtg tatcgataga tgtgtgaatg
961tggagggctg gcagccaaac atgatcttgg atgatggagg ggatcttacc cactggattt
1021ataaaaagta tcccaacatg tttaagaaaa tcaagggcat agtagaggag agtgttactg
1081gagttcacag gctgtaccaa ctgtccaaag ctgggaagct gtgtgttcca gccatgaatg
1141tcaatgactc agtcaccaaa cagaaatttg acaacctcta ctgttgccgt gaatcaattc
1201ttgatggact taaaaggaca acagacatga tgtttggtgg aaagcaagtg gtagtctgtg
1261gctatggaga ggtggggaaa gggtgctgtg ctgccctgaa agccatgggc tccattgtgt
1321atgtaactga aattgacccc atctgtgccc tgcaagcctg tatggatgga tttcgactgg
1381tgaaattaaa tgaggtcatc cgacaagtgg acattgttat tacctgtaca ggtaacaaga
1441atgtggtaac cagagagcac ttggaccgta tgaagaatag ctgcatcgtt tgtaacatgg
1501gacattccaa cacagagatt gacgtggcga gtctgcggac accagaactg acctgggagc
1561gagtgagatc tcaagttgac catgtgatat ggcctgatgg caagaggata gtactgctgg
1621cagagggccg cctgctgaac cttagctgct ccacagtgcc tacatttgtg ctctcaatca
1681ctgctactac tcaggctctt gccttgatag agctttacaa tgctcctgag ggtcgctata
1741agcaggatgt ctacctgttg cccaagaaga tggatgagta tgtggccagc ctacacctgc
1801ctacctttga tgcccacttg acagagctga cagatgaaca ggccaagtat ctgggactca
1861acaagaatgg gcccttcaag cctaattact acaggtatta cccaacttnc ttgtacaaag
1921tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1981gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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