Transcript: Human XM_017014577.1

PREDICTED: Homo sapiens ankyrin repeat domain 18A (ANKRD18A), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD18A (253650)
Length:
3270
CDS:
298..2424

Additional Resources:

NCBI RefSeq record:
XM_017014577.1
NBCI Gene record:
ANKRD18A (253650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014577.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000161866 GCAGCCATGAAGAATGAAAGT pLKO.1 1108 CDS 100% 4.950 2.970 N ANKRD18B n/a
2 TRCN0000159733 GCTAAAGAAAGTCAATCCATT pLKO.1 2068 CDS 100% 4.950 2.475 Y ANKRD18B n/a
3 TRCN0000138852 GCTCTCCATTATGCCGTGTAT pLKO.1 706 CDS 100% 4.950 2.475 Y ANKRD20A3 n/a
4 TRCN0000151931 CCATGCAAATATTGAAGCACT pLKO.1 765 CDS 100% 2.640 1.320 Y ANKRD19P n/a
5 TRCN0000157785 CAGATCGACATCTGTGACAGA pLKO.1 574 CDS 100% 0.264 0.132 Y ANKRD18DP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014577.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09894 pDONR223 100% 60.1% 59.2% None (many diffs) n/a
2 ccsbBroad304_09894 pLX_304 0% 60.1% 59.2% V5 (many diffs) n/a
3 TRCN0000475606 CCGGTTCTGATATGTTTTATAAAT pLX_317 11.9% 60.1% 59.2% V5 (many diffs) n/a
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