Transcript: Human XM_017018549.1

PREDICTED: Homo sapiens aminoacylase 3 (ACY3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACY3 (91703)
Length:
1495
CDS:
342..1322

Additional Resources:

NCBI RefSeq record:
XM_017018549.1
NBCI Gene record:
ACY3 (91703)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018549.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437010 GCTTCCTAACCCAAGACACAC pLKO_005 1314 CDS 100% 4.050 5.670 N ACY3 n/a
2 TRCN0000048771 CTTTGTCCTTGACCTGCACAA pLKO.1 692 CDS 100% 4.050 3.240 N ACY3 n/a
3 TRCN0000048768 CCAGACTGAGAAGTTCACATT pLKO.1 1250 CDS 100% 4.950 3.465 N ACY3 n/a
4 TRCN0000445642 AGCTACAACCTGGACTCTGTG pLKO_005 852 CDS 100% 4.050 2.835 N ACY3 n/a
5 TRCN0000048770 GCCTACTATGAGAAGGGCGTT pLKO.1 1221 CDS 100% 2.160 1.512 N ACY3 n/a
6 TRCN0000048772 CCATGACCTCAACCGCACCTT pLKO.1 539 CDS 100% 0.880 0.616 N ACY3 n/a
7 TRCN0000048769 GCCTTTGAGATGGAAGCCTAT pLKO.1 1011 CDS 100% 4.050 2.430 N ACY3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018549.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04557 pDONR223 100% 97.8% 97.5% None 237_257del n/a
2 ccsbBroad304_04557 pLX_304 0% 97.8% 97.5% V5 237_257del n/a
3 TRCN0000470463 CCTACATCGAAGCCCTCCCCTCCC pLX_317 34.1% 97.8% 97.5% V5 237_257del n/a
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