Transcript: Human XM_017018810.1

PREDICTED: Homo sapiens anoctamin 4 (ANO4), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANO4 (121601)
Length:
3635
CDS:
426..3188

Additional Resources:

NCBI RefSeq record:
XM_017018810.1
NBCI Gene record:
ANO4 (121601)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018810.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000248383 GAACTTGGCTACCCGTTAATT pLKO_005 2313 CDS 100% 15.000 21.000 N Ano4 n/a
2 TRCN0000248384 TTCATGAGCAGGATCGATAAA pLKO_005 912 CDS 100% 13.200 18.480 N Ano4 n/a
3 TRCN0000168808 GCGAGCAGTAATTGCTTATGA pLKO.1 1664 CDS 100% 5.625 7.875 N ANO4 n/a
4 TRCN0000172418 CCACAGTGCATGTTGCAGATA pLKO.1 3288 3UTR 100% 4.950 3.465 N ANO4 n/a
5 TRCN0000168400 GCTGTGTATATGCCAAGGTAA pLKO.1 1555 CDS 100% 4.950 3.465 N ANO4 n/a
6 TRCN0000167227 CACTTCATCATACACAACAAA pLKO.1 1053 CDS 100% 5.625 3.375 N ANO4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018810.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13086 pDONR223 100% 96.3% 96.2% None 55_56ins105 n/a
2 ccsbBroad304_13086 pLX_304 0% 96.3% 96.2% V5 55_56ins105 n/a
3 TRCN0000466450 CCACAAACCCTCTGGAGTAGCACA pLX_317 14.4% 96.3% 96.2% V5 55_56ins105 n/a
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