Construct: ORF TRCN0000466450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014177.1_s317c1
- Derived from:
- ccsbBroadEn_13086
- DNA Barcode:
- CCACAAACCCTCTGGAGTAGCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANO4 (121601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121601 | ANO4 | anoctamin 4 | NM_001286615.2 | 100% | 100% | |
| 2 | human | 121601 | ANO4 | anoctamin 4 | NM_001286616.1 | 100% | 100% | |
| 3 | human | 121601 | ANO4 | anoctamin 4 | NM_178826.4 | 96.3% | 96.2% | 55_56ins105 |
| 4 | human | 121601 | ANO4 | anoctamin 4 | XM_011537914.2 | 96.3% | 96.2% | 55_56ins105 |
| 5 | human | 121601 | ANO4 | anoctamin 4 | XM_017018808.1 | 96.3% | 96.2% | 55_56ins105 |
| 6 | human | 121601 | ANO4 | anoctamin 4 | XM_017018810.1 | 96.3% | 96.2% | 55_56ins105 |
| 7 | human | 121601 | ANO4 | anoctamin 4 | XM_011537916.2 | 95% | 95% | 1_150del |
| 8 | human | 121601 | ANO4 | anoctamin 4 | XM_017018811.2 | 94.7% | 94.6% | 55_56ins105;449_450ins45 |
| 9 | human | 121601 | ANO4 | anoctamin 4 | XM_017018812.2 | 93.8% | 93.7% | 557_558ins177 |
| 10 | human | 121601 | ANO4 | anoctamin 4 | XM_017018813.2 | 90.1% | 89.9% | 55_56ins105;452_453ins177 |
| 11 | human | 121601 | ANO4 | anoctamin 4 | XM_011537911.2 | 85.1% | 85.1% | 1_498del |
| 12 | human | 121601 | ANO4 | anoctamin 4 | XM_011537912.2 | 85.1% | 85.1% | 1_498del |
| 13 | human | 121601 | ANO4 | anoctamin 4 | XM_011537913.2 | 83.8% | 83.8% | 1_498del;1052_1053ins45 |
| 14 | human | 121601 | ANO4 | anoctamin 4 | XM_017018814.2 | 81.1% | 80.7% | (many diffs) |
| 15 | human | 121601 | ANO4 | anoctamin 4 | XM_017018815.2 | 81.1% | 80.7% | (many diffs) |
| 16 | human | 121601 | ANO4 | anoctamin 4 | XM_017018817.2 | 81.1% | 80.7% | (many diffs) |
| 17 | human | 121601 | ANO4 | anoctamin 4 | XM_024448841.1 | 81.1% | 80.7% | (many diffs) |
| 18 | human | 121601 | ANO4 | anoctamin 4 | XM_024448842.1 | 81.1% | 80.7% | (many diffs) |
| 19 | human | 121601 | ANO4 | anoctamin 4 | XM_011537915.2 | 79.9% | 79.8% | 1_498del;1055_1056ins177 |
| 20 | human | 121601 | ANO4 | anoctamin 4 | XM_011537918.2 | 39.7% | 39.2% | (many diffs) |
| 21 | mouse | 320091 | Ano4 | anoctamin 4 | NM_001277188.1 | 92% | 98.3% | (many diffs) |
| 22 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513758.3 | 92% | 98.3% | (many diffs) |
| 23 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513759.3 | 88.7% | 94.8% | (many diffs) |
| 24 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513755.3 | 76.1% | 81.3% | (many diffs) |
| 25 | mouse | 320091 | Ano4 | anoctamin 4 | XM_011243478.2 | 74.9% | 80% | (many diffs) |
| 26 | mouse | 320091 | Ano4 | anoctamin 4 | XM_017313966.1 | 74.9% | 80% | (many diffs) |
| 27 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513756.3 | 74.9% | 80% | (many diffs) |
| 28 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513757.3 | 73.4% | 78.5% | (many diffs) |
| 29 | mouse | 320091 | Ano4 | anoctamin 4 | NR_102339.1 | 59.9% | (many diffs) | |
| 30 | mouse | 320091 | Ano4 | anoctamin 4 | XM_006513760.3 | 55.6% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2934
- ORF length:
- 2865
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcaagc tcttctggaa tcactaatgg aaaaaccaaa gtcttccacc 121 cagaaggagg tgtggatttg caaggctacc agctggatat gcaaatacta cctgacgggc 181 caaagagtga tgtggacttt tcagagattc ttaatgcaat acaagaaatg gccaaggatg 241 tcaatattct ttttgatgaa ttagaagctg tcagcagtcc ttgcaaagat gacgattctc 301 ttcttcaccc tggaaacctg actagtactt cagatgatgc cagcagattg gaagccgggg 361 gagagacagt gccagaaaga aacaaatcaa atggacttta ctttcgagat ggaaagtgtc 421 gaattgacta catccttgtg tacagaaaat ccaaccccca gactgaaaag agagaagtat 481 ttgaaagaaa cattagagca gaaggattgc aaatggagaa agagtcctct ctaataaata 541 gtgacattat ctttgtgaag ttgcatgccc catgggaagt ccttggaaga tatgcagaac 601 aaatgaatgt aagaatgcct ttcaggagaa aaatctatta cctgccccgc cgttacaagt 661 tcatgagcag gatcgataaa caaataagca ggtttcggag atggttacct aagaagccaa 721 tgaggctgga caaggagaca ctgccagacc tggaggagaa tgactgctac actgcccctt 781 tcagccagca aaggatccat cacttcatca tacacaacaa agaaacgttc ttcaacaatg 841 ccacaagaag tagaatcgtg catcacattt tacaaagaat aaaatatgaa gaaggaaaaa 901 acaagattgg tctgaatcgt ttgcttacca atggctccta tgaagctgcg tttcccctgc 961 atgagggaag ttatagaagt aaaaactcca ttcgaaccca tggagcagaa aaccaccgac 1021 atctactcta tgagtgctgg gcctcctggg gcgtgtggta taaataccaa cctttggatc 1081 ttgtaaggcg gtactttgga gagaagattg ggttatattt tgcctggttg ggctggtaca 1141 ccggcatgct cttcccagct gccttcattg gattgtttgt ctttttgtat ggcgtcacca 1201 ctctggatca cagccaagtc agtaaagaag tctgccaagc tacagatatc atcatgtgtc 1261 ctgtgtgtga taaatactgt ccattcatga ggctgtcaga cagctgtgta tatgccaagg 1321 taacccacct ttttgacaat ggagccactg tcttctttgc tgttttcatg gcagtctggg 1381 caacagtttt cctggagttt tggaaaagac ggcgagcagt aattgcttat gactgggatt 1441 tgatagactg ggaagaagag gaggaagaaa tacgacccca gtttgaagcc aagtattcca 1501 agaaagagcg gatgaatcca atttctggaa agccagaacc ttatcaagca tttacagata 1561 aatgcagcag acttatcgtt tctgcatctg gaatattttt tatgatctgc gtggtgattg 1621 ctgccgtgtt cgggatcgtc atttaccggg tggtgactgt cagcactttc gctgccttta 1681 agtgggcgtt aatcaggaat aactctcagg ttgcaaccac agggactgct gtgtgcatca 1741 acttctgtat cattatgttg ctgaatgtgc tctatgaaaa agttgccctg cttctgacga 1801 atttagaaca gcctcgcaca gagtctgagt gggagaacag cttcaccctg aaaatgtttc 1861 tttttcagtt tgtcaatctg aacagctcca cattttacat cgcattcttc ctcggaagat 1921 ttacaggaca cccaggtgcc tacttgaggc tgataaacag gtggagacta gaagagtgcc 1981 accctagtgg atgccttatt gatctgtgta tgcaaatggg tattataatg gtgctaaagc 2041 agacctggaa taatttcatg gaacttggct acccgttaat tcagaattgg tggactagaa 2101 gaaaagtacg acaagaacat ggacctgaaa ggaaaataag tttcccacaa tgggaaaagg 2161 actataacct tcagccgatg aatgcctatg gactcttcga tgaatactta gaaatgattc 2221 ttcagtttgg attcacaact atctttgtgg cagcttttcc cctagcacca cttctggcct 2281 tactgaataa cataattgaa attcgacttg atgcttacaa atttgtcaca cagtggagga 2341 gacctttagc ttcaagggcc aaagacatag gaatttggta tggaattctt gaaggcattg 2401 gaattctctc tgttatcaca aatgcatttg tcatagcgat aacatctgac tttatccctc 2461 gcttggtgta tgcttataag tatggacctt gtgcaggcca aggagaagct gggcaaaagt 2521 gcatggttGG CTATGTGAAT GCCAGCTTGT CTGTATTTCG AATTTCTGAC TTTGAGAACC 2581 GATCTGAGCC TGAATCTGAT GGCAGTGAGT TCTCGGGGAC TCCTCTTAAG TACTGCAGAT 2641 ACCGGGACTA CCGTGACCCG CCTCATTCAC TGGTGCCCTA TGGCTACACA CTGCAGTTTT 2701 GGCATGTCCT AGCTGCTCGA TTAGCTTTTA TCATTGTCTT TGAGCACCTC GTGTTTTGTA 2761 TAAAGCACCT CATTTCATAT CTGATCCCAG ACCTCCCAAA AGACCTAAGG GATCGAATGA 2821 GAAGAGAGAA GTACTTGATT CAGGAGATGA TGTATGAAGC AGAACTGGAA CGTCTCCAGA 2881 AGGAACGAAA GGAGAGGAAG AAGAATGGAA AAGCACACCA CAACGAGTGG CCGTTGCCAA 2941 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3001 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3061 TATCTTGTGG AAAGGACGAC CACAAACCCT CTGGAGTAGC ACAACGCGTT AAGTCgacaa 3121 tcaacctctg gattacaaaa tttgtgaaag att