Transcript: Human XM_017019033.1

PREDICTED: Homo sapiens R3H domain containing 2 (R3HDM2), transcript variant X32, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
R3HDM2 (22864)
Length:
3357
CDS:
435..2348

Additional Resources:

NCBI RefSeq record:
XM_017019033.1
NBCI Gene record:
R3HDM2 (22864)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017019033.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380877 CAGTATGGACCGAGATGATAA pLKO_005 107 5UTR 100% 13.200 18.480 N R3HDM2 n/a
2 TRCN0000236193 TGGCCAGAACGGATATCTAAA pLKO_005 284 5UTR 100% 13.200 18.480 N R3HDM2 n/a
3 TRCN0000236189 AGCGAGCCAGCTCCCAATAAA pLKO_005 2329 CDS 100% 15.000 12.000 N R3HDM2 n/a
4 TRCN0000236191 AGAGGTTCTCAGAACATATAA pLKO_005 31 5UTR 100% 15.000 10.500 N R3HDM2 n/a
5 TRCN0000413475 AGAGGTTCTCAGAACATATAA pLKO_005 31 5UTR 100% 15.000 10.500 N R3hdm2 n/a
6 TRCN0000082650 CCAGTGGAGTCATTGTAAATA pLKO.1 1592 CDS 100% 15.000 10.500 N R3HDM2 n/a
7 TRCN0000236190 AGTAAGGAAACTGATGTTAAA pLKO_005 2439 3UTR 100% 13.200 9.240 N R3HDM2 n/a
8 TRCN0000082649 GCAAGAGACAAGCACTCAAAT pLKO.1 1954 CDS 100% 13.200 9.240 N R3HDM2 n/a
9 TRCN0000082652 GCGGCTTACCAAGGCATGATT pLKO.1 1122 CDS 100% 5.625 3.938 N R3HDM2 n/a
10 TRCN0000082651 CCCTCAGCAGACTAGCTTCAT pLKO.1 830 CDS 100% 4.950 3.465 N R3HDM2 n/a
11 TRCN0000082648 CCGCAGTAACAGACACAGTAT pLKO.1 3202 3UTR 100% 4.950 3.465 N R3HDM2 n/a
12 TRCN0000080594 GCCACCCTTGAATAATCACAT pLKO.1 689 CDS 100% 4.950 3.465 N R3hdm2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017019033.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15744 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15744 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470723 GCTAGCGGACTCCGTGCTTCTGCT pLX_317 20% 100% 100% V5 n/a
4 TRCN0000469031 ATCCTAATTAAACGCCGTGTGGCG pLX_317 18.8% 99.6% 99.6% V5 (many diffs) n/a
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