Transcript: Human XM_017020314.2

PREDICTED: Homo sapiens ubiquitin specific peptidase like 1 (USPL1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
USPL1 (10208)
Length:
4802
CDS:
238..3402

Additional Resources:

NCBI RefSeq record:
XM_017020314.2
NBCI Gene record:
USPL1 (10208)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017020314.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000314831 GTCCGATGAAGACTGATATTT pLKO_005 3287 CDS 100% 15.000 21.000 N USPL1 n/a
2 TRCN0000007514 CGTCAAACTTACCTGATAGTA pLKO.1 686 CDS 100% 5.625 7.875 N USPL1 n/a
3 TRCN0000350388 CGTCAAACTTACCTGATAGTA pLKO_005 686 CDS 100% 5.625 7.875 N USPL1 n/a
4 TRCN0000007511 CCCATATTCATGTTGCACTTT pLKO.1 1369 CDS 100% 4.950 6.930 N USPL1 n/a
5 TRCN0000007512 CCTCAGTTTCACAGTTAAATT pLKO.1 1910 CDS 100% 15.000 10.500 N USPL1 n/a
6 TRCN0000314830 CCTCAGTTTCACAGTTAAATT pLKO_005 1910 CDS 100% 15.000 10.500 N USPL1 n/a
7 TRCN0000314833 GTTGGGTTAAAGGCTTAATAA pLKO_005 2396 CDS 100% 15.000 10.500 N USPL1 n/a
8 TRCN0000007513 CCTACCTCATTTCGATGAATA pLKO.1 3363 CDS 100% 13.200 9.240 N USPL1 n/a
9 TRCN0000314777 GCTCGAAGGAGGAATCTATAT pLKO_005 1013 CDS 100% 13.200 9.240 N USPL1 n/a
10 TRCN0000007510 ACAATGTGTTCAGGTAGTGTT pLKO.1 3456 3UTR 100% 4.950 3.465 N USPL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017020314.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07568 pDONR223 100% 96.4% 96.3% None (many diffs) n/a
2 ccsbBroad304_07568 pLX_304 0% 96.4% 96.3% V5 (many diffs) n/a
3 TRCN0000474590 GATAAGTATAGGCCAAAGGACAGG pLX_317 16.3% 96.4% 96.3% V5 (many diffs) n/a
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