Construct: ORF ccsbBroad304_07568
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013535.1_s304c1, BRDN0000407212
- Derived from:
- ccsbBroadEn_07568
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USPL1 (10208)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07568
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10208 | USPL1 | ubiquitin specific peptidas... | NM_005800.5 | 99.9% | 99.8% | 627A>G;1592T>C;2849G>A |
| 2 | human | 10208 | USPL1 | ubiquitin specific peptidas... | XM_006719751.4 | 99.3% | 99.2% | (many diffs) |
| 3 | human | 10208 | USPL1 | ubiquitin specific peptidas... | XM_017020314.2 | 96.4% | 96.3% | (many diffs) |
| 4 | human | 10208 | USPL1 | ubiquitin specific peptidas... | NM_001321532.2 | 83.3% | 83.2% | (many diffs) |
| 5 | human | 10208 | USPL1 | ubiquitin specific peptidas... | XM_017020315.1 | 79.8% | 79.7% | (many diffs) |
| 6 | human | 10208 | USPL1 | ubiquitin specific peptidas... | NM_001321533.2 | 69.8% | 69.6% | 0_1ins987;605T>C;1862G>A |
| 7 | human | 10208 | USPL1 | ubiquitin specific peptidas... | NM_001321534.2 | 69.8% | 69.6% | 0_1ins987;605T>C;1862G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3342
- ORF length:
- 3276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat ggattctccg aagattggaa atggtttgcc agtgattgga ccagggactg 121 atatagggat atcttcactc cacatggtgg ggtatttggg aaaaaatttt gattcagcta 181 aagttccatc agatgagtat tgccctgctt gtagagagaa gggaaagtta aaagccttaa 241 agacttaccg aattagtttt caagaatcta tctttttgtg tgaggatctg cagtgcatct 301 atcctttggg ctctaaatca cttaataacc taatttctcc tgatttggaa gaatgtcaca 361 ctccacataa gcctcagaaa aggaagagct tagaaagcag ctataaggat tcacttcttt 421 tagcaaattc caaaaagact agaaattata ttgctattga cggtggaaaa gttttgaaca 481 gcaaacataa tggagaagta tatgacgaaa cctcgtcaaa cttacctgat agtagtggtc 541 aacagaatcc aattaggaca gctgattcct tggagcggaa tgagattttg gaagctgata 601 ctgttgacat ggctactaca aaagatcctg ctacagttga tgtctctgga actggcagac 661 cttcccctca aaatgaagga tgtacatcta agctggaaat gccactggag agcaaatgta 721 catcatttcc ccaggcttta tgtgtccagt ggaaaaatgc ttatgctctc tgttggttag 781 actgtatcct gtcagctttg gtgcactcgg aagagttaaa gaacaccgtg actggactgt 841 gctcgaagga ggaatctata ttctggcggt tgcttacaaa atataatcaa gcaaatacac 901 ttctatatac cagtcaattg agtggtgtta aagatggaga ttgtaaaaaa cttacctcag 961 aaatatttgc agagatagag acctgtctga atgaagttag agatgaaatt tttattagcc 1021 ttcagcccca gcttagatgc acattaggtg atatggaaag ccctgtgttt gcatttcccc 1081 tgctcttaaa actagaaacc cacattgaaa agctcttcct atattctttt tcttgggact 1141 ttgaatgttc gcagtgtgga caccaatatc aaaacaggca tatgaagagt ctggtcacct 1201 ttacaaatgt catccctgag tggcacccac ttaatgctgc ccattttggt ccatgtaaca 1261 attgcaacag taaatcacaa ataagaaaaa tggtattaga aaaagtatct cccatattca 1321 tgttgcactt tgtagaaggc ttaccacaga atgacttgca gcactatgca tttcattttg 1381 aaggctgtct ttatcagata acttctgtaa ttcagtatcg agcaaataat cattttataa 1441 catggatttt agatgctgat ggaagttggc tggaatgtga tgacttaaaa ggcccatgtt 1501 ctgaaaggca caagaaattt gaagttcctg cttcagagat acatattgtt atttgggaaa 1561 gaaaaatatc ccaagtgaca gataaagaag ctgcctgcct tccacttaaa aagactaatg 1621 accaacacgc tctcagtaat gagaaaccag tatcttcaac atcgtgttct gtgggtgatg 1681 ctgcctcagc tgaaacagcc tcagtaactc accctaaaga tatatcagtt gcccctcgta 1741 ctctttcaca ggacacagct gtaactcatg gagatcattt actttcaggt ccaaaaggtt 1801 tggttgacaa tattttacct ctgacacttg aagaaactat ccagaaaaca gcctcagttt 1861 cacagttaaa ttctgaagct ttcctgttag aaaataaacc tgtagcagaa aatacaggaa 1921 ttctcaaaac caatactttg ctatcacaag aatcactaat ggcttcttca gtatcagctc 1981 catgtaatga aaagcttatt caagaccaat ttgtggacat aagttttcca tcccaagttg 2041 taaatacaaa catgcagtca gtacagctga atacagaaga tactgtaaat actaaatctg 2101 tgaataatac tgatgctact ggtcttatac agggagtgaa gtcagtagaa attgagaagg 2161 acgctcagtt aaaacaattc cttacaccaa aaactgaaca attaaaacca gaacgtgtca 2221 catctcaggt atctaatttg aagaaaaaag aaactacagc agattctcaa accacaacat 2281 ctaagtcatt acagaatcag tctctgaaag aaaatcagaa gaagccattt gtgggaagtt 2341 gggttaaagg cttaataagc aggggtgctt cttttatgcc actctgtgtt tcagctcata 2401 atagaaacac tataactgat ttacaacctt cagttaaagg ggtaaataat tttggtggct 2461 ttaaaactaa aggtataaac cagaaggcca gccacgtatc caagaaagct cgtaagagtg 2521 caagtaagcc tcctcccatc agtaagccac cagcaggccc tccatcgtct aatggcacag 2581 ctgcccaccc acatgctcat gctgcttcag aagttttgga aaagtctgga agcacctcat 2641 gtggagctca actcaaccac agttcttatg ggaatggtat ttcttcagca aaccatgaag 2701 acttggtgga aggtcagatt cataaacttc gtctaaaact tcgtaaaaag ctaaaggcag 2761 aaaagaagaa attagctgct cttatgtctt ccccgcaaag cagaacagtt cgaagtgaaa 2821 atctagaaca ggtgccccag gatgggtctc caaatgattg tgaatcaata gaggacttgt 2881 taaatgagct accatatcca attgatattg ccaatgagtc tgcatgcacc actgttcctg 2941 gtgtttccct gtacagtagt caaactcatg aagaaatttt agcggaatta ttgtctccta 3001 cacctgtttc aacagagctg tcagaaaatg gggaaggtga ctttaggtat ttgggaatgg 3061 gagatagtca tatcccacca ccagtaccaa gtgaattcaa tgatgtttcc cagaacacac 3121 atctgagaca ggaccataat tattgtagcc ccaccaagaa aaatccatgt gaagttcagc 3181 cagactctct gacaaataat gcctgcgtta gaacattaaa cttggagagt ccgatgaaga 3241 ctgatatttt cgatgagttt ttttcctcct cagcattaaa tgctttagca aatgacacat 3301 tagacctacc tcatttcgat gaatatctgt ttgagaatta ttgcccaact ttcttgtaca 3361 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3421 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta