Transcript: Human XM_017020874.1

PREDICTED: Homo sapiens mitochondrial translation release factor 1 (MTRF1), transcript variant X29, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MTRF1 (9617)
Length:
2287
CDS:
305..1642

Additional Resources:

NCBI RefSeq record:
XM_017020874.1
NBCI Gene record:
MTRF1 (9617)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017020874.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232959 GATTTGCGAATAGATACATTT pLKO_005 1199 CDS 100% 13.200 18.480 N MTRF1 n/a
2 TRCN0000154320 CCAGCAGATTATGGTGGACTA pLKO.1 989 CDS 100% 4.050 5.670 N MTRF1 n/a
3 TRCN0000155034 GCTAGACTCTACCAGCAGATT pLKO.1 1364 CDS 100% 4.950 3.960 N MTRF1 n/a
4 TRCN0000232958 CAGAATTATTCGTGCTATAAA pLKO_005 938 CDS 100% 15.000 10.500 N MTRF1 n/a
5 TRCN0000232957 CCAACAATTTACCCGAGAAAT pLKO_005 904 CDS 100% 13.200 9.240 N MTRF1 n/a
6 TRCN0000232960 TAAATGAAATGGACCTATATC pLKO_005 1672 3UTR 100% 13.200 9.240 N MTRF1 n/a
7 TRCN0000152306 CCAACAAGAAAGATCACAGAT pLKO.1 1309 CDS 100% 4.950 3.465 N MTRF1 n/a
8 TRCN0000150642 GACAAGTCACTTGTTCTCTTT pLKO.1 1914 3UTR 100% 4.950 3.465 N MTRF1 n/a
9 TRCN0000154737 GCATTCACACAGGAACGATGT pLKO.1 1125 CDS 100% 4.050 2.835 N MTRF1 n/a
10 TRCN0000155064 GCCTGGATCAGCTAATTCAGA pLKO.1 1551 CDS 100% 3.000 2.100 N MTRF1 n/a
11 TRCN0000153013 GAAGTCACATTTCCTGACCTA pLKO.1 1787 3UTR 100% 2.640 1.848 N MTRF1 n/a
12 TRCN0000257053 TTTCCGGTGACGGTGTCTATA pLKO_005 1029 CDS 100% 13.200 7.920 N MTRF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017020874.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11394 pDONR223 100% 32.5% 31.2% None (many diffs) n/a
2 ccsbBroad304_11394 pLX_304 0% 32.5% 31.2% V5 (many diffs) n/a
3 TRCN0000480237 GAGAGCAACCATCCTGTAATGGCC pLX_317 76.7% 32.5% 31.2% V5 (many diffs) n/a
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