Construct: ORF TRCN0000480237

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF015962.1_s317c1
• Derived from: ccsbBroadEn_11394
• DNA Barcode: GAGAGCAACCATCCTGTAATGGCC
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: MTRF1 (9617)

Vector Information:

• Vector Backbone: pLX_317
• Pol II Cassette 1: SV40-PuroR
• Pol II Cassette 2: EF1a-TRCN0000480237
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	9617	MTRF1	mitochondrial translation r...	XM_017020879.2	56%	54%	(many diffs)
2	human	9617	MTRF1	mitochondrial translation r...	XM_024449444.1	56%	54%	(many diffs)
3	human	9617	MTRF1	mitochondrial translation r...	XM_011535327.2	46.7%	44.9%	(many diffs)
4	human	9617	MTRF1	mitochondrial translation r...	XM_017020878.2	43.5%	41.8%	(many diffs)
5	human	9617	MTRF1	mitochondrial translation r...	XM_011535326.3	37.6%	36.1%	(many diffs)
6	human	9617	MTRF1	mitochondrial translation r...	XM_017020872.1	37%	35.6%	(many diffs)
7	human	9617	MTRF1	mitochondrial translation r...	XR_001749741.1	33.4%		(many diffs)
8	human	9617	MTRF1	mitochondrial translation r...	XM_017020870.2	32.6%	31.3%	(many diffs)
9	human	9617	MTRF1	mitochondrial translation r...	XM_017020871.1	32.6%	31.3%	(many diffs)
10	human	9617	MTRF1	mitochondrial translation r...	NM_001354073.1	32.5%	31.2%	(many diffs)
11	human	9617	MTRF1	mitochondrial translation r...	NM_001354074.1	32.5%	31.2%	(many diffs)
12	human	9617	MTRF1	mitochondrial translation r...	NM_001354076.1	32.5%	31.2%	(many diffs)
13	human	9617	MTRF1	mitochondrial translation r...	NM_004294.4	32.5%	31.2%	(many diffs)
14	human	9617	MTRF1	mitochondrial translation r...	XM_006719898.2	32.5%	31.2%	(many diffs)
15	human	9617	MTRF1	mitochondrial translation r...	XM_006719900.2	32.5%	31.2%	(many diffs)
16	human	9617	MTRF1	mitochondrial translation r...	XM_011535318.3	32.5%	31.2%	(many diffs)
17	human	9617	MTRF1	mitochondrial translation r...	XM_011535320.2	32.5%	31.2%	(many diffs)
18	human	9617	MTRF1	mitochondrial translation r...	XM_011535321.2	32.5%	31.2%	(many diffs)
19	human	9617	MTRF1	mitochondrial translation r...	XM_011535323.2	32.5%	31.2%	(many diffs)
20	human	9617	MTRF1	mitochondrial translation r...	XM_011535325.2	32.5%	31.2%	(many diffs)
21	human	9617	MTRF1	mitochondrial translation r...	XM_017020865.2	32.5%	31.2%	(many diffs)
22	human	9617	MTRF1	mitochondrial translation r...	XM_017020866.2	32.5%	31.2%	(many diffs)
23	human	9617	MTRF1	mitochondrial translation r...	XM_017020867.1	32.5%	31.2%	(many diffs)
24	human	9617	MTRF1	mitochondrial translation r...	XM_017020868.1	32.5%	31.2%	(many diffs)
25	human	9617	MTRF1	mitochondrial translation r...	XM_017020869.2	32.5%	31.2%	(many diffs)
26	human	9617	MTRF1	mitochondrial translation r...	XM_017020874.1	32.5%	31.2%	(many diffs)
27	human	9617	MTRF1	mitochondrial translation r...	XM_017020875.1	32.5%	31.2%	(many diffs)
28	human	9617	MTRF1	mitochondrial translation r...	XM_024449440.1	32.5%	31.2%	(many diffs)
29	human	9617	MTRF1	mitochondrial translation r...	XM_024449441.1	32.5%	31.2%	(many diffs)
30	human	9617	MTRF1	mitochondrial translation r...	XM_024449442.1	32.5%	31.2%	(many diffs)
31	human	9617	MTRF1	mitochondrial translation r...	XM_024449443.1	32.5%	31.2%	(many diffs)
32	human	9617	MTRF1	mitochondrial translation r...	XM_017020861.2	31.6%	30.4%	(many diffs)
33	human	9617	MTRF1	mitochondrial translation r...	XM_011535317.2	31.6%	30.3%	(many diffs)
34	human	9617	MTRF1	mitochondrial translation r...	XM_017020860.1	31.6%	30.3%	(many diffs)
35	human	9617	MTRF1	mitochondrial translation r...	XM_017020859.2	29.2%	28%	(many diffs)
36	human	9617	MTRF1	mitochondrial translation r...	XM_011535315.2	29.1%	27.9%	(many diffs)
37	human	9617	MTRF1	mitochondrial translation r...	XM_017020856.1	29.1%	27.9%	(many diffs)
38	human	9617	MTRF1	mitochondrial translation r...	XM_017020857.1	29.1%	27.9%	(many diffs)
39	human	9617	MTRF1	mitochondrial translation r...	XM_017020858.1	29.1%	27.9%	(many diffs)
40	human	9617	MTRF1	mitochondrial translation r...	XR_001749743.1	17.8%		(many diffs)
41	human	9617	MTRF1	mitochondrial translation r...	XR_001749744.2	16%		(many diffs)
42	human	9617	MTRF1	mitochondrial translation r...	XR_002957484.1	11.9%		(many diffs)
43	human	9617	MTRF1	mitochondrial translation r...	XR_001749742.2	11.3%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

519

ORF length:

453

Sequence:

1	tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61	ttggcatgaa tcgtcacctg tgtgtttggc tttttagaca tccatctctt aatggttacc
121	tccagtgtca catccagctc cattctcatc aatttagaca gatacaTCTT GATACAAGGC
181	TGCAAGTTTT TAGACAAAAC AGGAATTGCA TTCTTCATCT GTTAAGTAAG AATTGGTCCA
241	GGAGATATTG CCATCAAGAC ACCAAGATGC TCTGGAAGCA TAAAGCACTA CAGAAATATA
301	TGGAGAACCT GAGTAAGGAG TACCAAACAC TTGAGCAATG TCTGCAGCAT ATCCCTGTGA
361	ATGAGGAAAA CCGAAGGTCC TTGAACAGAA GGCATGCTGA GTTGGCACCT CTTGCAGCCA
421	TTTACCAAGA AATTCAGGAG ACTGAACAAG CAATTGAAGA ATTAGAATCA ATGTGTAAAA
481	AGACAGAGTC TTGCTCTGTG GCCCAGGCTG GAATGCAGTA CCCAACTTTC TTGTACAAAG
541	TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
601	GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
661	ACGAGAGAGC AACCATCCTG TAATGGCCAC GCGTTAAGTC gacaatcaac ctctggatta
721	caaaatttgt gaaagatt

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