Transcript: Human XM_017022766.1

PREDICTED: Homo sapiens ubiquitin specific peptidase 3 (USP3), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
USP3 (9960)
Length:
5783
CDS:
669..1976

Additional Resources:

NCBI RefSeq record:
XM_017022766.1
NBCI Gene record:
USP3 (9960)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022766.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000320690 GTTGATACATACGTAGAATTT pLKO_005 1683 CDS 100% 13.200 18.480 N USP3 n/a
2 TRCN0000320691 TACTGTTGTCACGGCTATATT pLKO_005 1334 CDS 100% 15.000 10.500 N USP3 n/a
3 TRCN0000007604 GCTGCCTTTCCACAGCTATAA pLKO.1 2369 3UTR 100% 13.200 9.240 N USP3 n/a
4 TRCN0000320763 GCTGCCTTTCCACAGCTATAA pLKO_005 2369 3UTR 100% 13.200 9.240 N USP3 n/a
5 TRCN0000007606 CCAACCATAAGAAATCAGAAA pLKO.1 619 5UTR 100% 4.950 3.465 N USP3 n/a
6 TRCN0000320694 CCAACCATAAGAAATCAGAAA pLKO_005 619 5UTR 100% 4.950 3.465 N USP3 n/a
7 TRCN0000007605 GCCTCATATGTGGGACAGAAT pLKO.1 1384 CDS 100% 0.000 0.000 N USP3 n/a
8 TRCN0000320688 GCCTCATATGTGGGACAGAAT pLKO_005 1384 CDS 100% 0.000 0.000 N USP3 n/a
9 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 345 5UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022766.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02275 pDONR223 100% 83.6% 83.6% None 0_1ins255 n/a
2 ccsbBroad304_02275 pLX_304 0% 83.6% 83.6% V5 0_1ins255 n/a
3 TRCN0000469117 AATTGGACTGAGCGCTCAGCCACA pLX_317 23.7% 83.6% 83.6% V5 0_1ins255 n/a
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