Transcript: Human XM_017023368.2

PREDICTED: Homo sapiens differentially expressed in FDCP 8 homolog (DEF8), transcript variant X19, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DEF8 (54849)
Length:
3989
CDS:
537..1892

Additional Resources:

NCBI RefSeq record:
XM_017023368.2
NBCI Gene record:
DEF8 (54849)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017023368.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000129263 CCAAGCTGAATACGAACTGAA pLKO.1 1124 CDS 100% 4.950 6.930 N DEF8 n/a
2 TRCN0000423251 TTGTGGAGAACGACGAGATGT pLKO_005 1558 CDS 100% 4.950 6.930 N DEF8 n/a
3 TRCN0000425593 CACCTGCACAGGGTGTTATTA pLKO_005 1040 CDS 100% 15.000 10.500 N DEF8 n/a
4 TRCN0000146295 CCATTCCGAAATCTGTGTAAT pLKO.1 3645 3UTR 100% 13.200 9.240 N DEF8 n/a
5 TRCN0000148418 CAATGAGGATGAGCCAAACAT pLKO.1 914 CDS 100% 5.625 3.938 N DEF8 n/a
6 TRCN0000130316 GCCATTCCGAAATCTGTGTAA pLKO.1 3644 3UTR 100% 4.950 3.465 N DEF8 n/a
7 TRCN0000129531 GCTCAGAAATGACCAGCCAAT pLKO.1 2817 3UTR 100% 4.050 2.835 N DEF8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017023368.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03468 pDONR223 100% 41.3% 37.2% None (many diffs) n/a
2 ccsbBroad304_03468 pLX_304 0% 41.3% 37.2% V5 (many diffs) n/a
3 TRCN0000471991 CACGAACCGTACTAAAGGATGGAT pLX_317 59.7% 41.3% 37.2% V5 (many diffs) n/a
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