Construct: ORF TRCN0000471991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007550.1_s317c1
- Derived from:
- ccsbBroadEn_03468
- DNA Barcode:
- CACGAACCGTACTAAAGGATGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEF8 (54849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54849 | DEF8 | differentially expressed in... | NM_001242821.1 | 100% | 100% | |
| 2 | human | 54849 | DEF8 | differentially expressed in... | NM_001242822.2 | 100% | 100% | |
| 3 | human | 54849 | DEF8 | differentially expressed in... | NM_017702.4 | 100% | 100% | |
| 4 | human | 54849 | DEF8 | differentially expressed in... | NM_001242819.1 | 42.9% | 38.6% | (many diffs) |
| 5 | human | 54849 | DEF8 | differentially expressed in... | XM_005256318.3 | 42.9% | 38.6% | (many diffs) |
| 6 | human | 54849 | DEF8 | differentially expressed in... | XM_017023360.2 | 42.9% | 38.6% | (many diffs) |
| 7 | human | 54849 | DEF8 | differentially expressed in... | XM_017023369.2 | 42.9% | 38.6% | (many diffs) |
| 8 | human | 54849 | DEF8 | differentially expressed in... | XM_011523158.2 | 41.5% | 37.4% | (many diffs) |
| 9 | human | 54849 | DEF8 | differentially expressed in... | XM_017023359.2 | 41.5% | 37.4% | (many diffs) |
| 10 | human | 54849 | DEF8 | differentially expressed in... | NM_001242818.2 | 41.3% | 37.2% | (many diffs) |
| 11 | human | 54849 | DEF8 | differentially expressed in... | NM_001242820.1 | 41.3% | 37.2% | (many diffs) |
| 12 | human | 54849 | DEF8 | differentially expressed in... | XM_017023358.2 | 41.3% | 37.2% | (many diffs) |
| 13 | human | 54849 | DEF8 | differentially expressed in... | XM_017023367.2 | 41.3% | 37.2% | (many diffs) |
| 14 | human | 54849 | DEF8 | differentially expressed in... | XM_017023368.2 | 41.3% | 37.2% | (many diffs) |
| 15 | human | 54849 | DEF8 | differentially expressed in... | XM_011523156.3 | 40.6% | 36.6% | (many diffs) |
| 16 | human | 54849 | DEF8 | differentially expressed in... | XM_011523157.3 | 40.6% | 36.6% | (many diffs) |
| 17 | human | 54849 | DEF8 | differentially expressed in... | XM_011523162.3 | 40.6% | 36.6% | (many diffs) |
| 18 | human | 54849 | DEF8 | differentially expressed in... | XM_017023366.2 | 40.6% | 36.6% | (many diffs) |
| 19 | human | 54849 | DEF8 | differentially expressed in... | XM_017023364.2 | 39.8% | 35.9% | (many diffs) |
| 20 | human | 54849 | DEF8 | differentially expressed in... | NM_001242816.2 | 39.1% | 35.1% | (many diffs) |
| 21 | human | 54849 | DEF8 | differentially expressed in... | XM_017023362.2 | 38.6% | 34.8% | (many diffs) |
| 22 | human | 54849 | DEF8 | differentially expressed in... | XM_017023361.2 | 38.4% | 34.7% | (many diffs) |
| 23 | human | 54849 | DEF8 | differentially expressed in... | XM_011523159.3 | 37.8% | 34.1% | (many diffs) |
| 24 | human | 54849 | DEF8 | differentially expressed in... | XM_017023365.1 | 37.6% | 34% | (many diffs) |
| 25 | human | 54849 | DEF8 | differentially expressed in... | NM_207514.3 | 36.4% | 32.9% | (many diffs) |
| 26 | human | 54849 | DEF8 | differentially expressed in... | XM_017023363.1 | 36.4% | 32.9% | (many diffs) |
| 27 | human | 54849 | DEF8 | differentially expressed in... | XM_011523160.3 | 35.8% | 32.4% | (many diffs) |
| 28 | human | 54849 | DEF8 | differentially expressed in... | XM_011523161.3 | 35.8% | 32.4% | (many diffs) |
| 29 | human | 54849 | DEF8 | differentially expressed in... | NM_001242817.2 | 28.1% | 24.4% | (many diffs) |
| 30 | mouse | 23854 | Def8 | differentially expressed in... | NM_001281803.1 | 37.3% | 33.4% | (many diffs) |
| 31 | mouse | 23854 | Def8 | differentially expressed in... | NM_054046.5 | 37.3% | 33.4% | (many diffs) |
| 32 | mouse | 23854 | Def8 | differentially expressed in... | XM_006530977.3 | 37.3% | 33.4% | (many diffs) |
| 33 | mouse | 23854 | Def8 | differentially expressed in... | XM_006530978.3 | 37.3% | 33.4% | (many diffs) |
| 34 | mouse | 23854 | Def8 | differentially expressed in... | NM_001253784.1 | 36.3% | 32.5% | (many diffs) |
| 35 | mouse | 23854 | Def8 | differentially expressed in... | XM_006530976.3 | 36.3% | 32.5% | (many diffs) |
| 36 | mouse | 23854 | Def8 | differentially expressed in... | NM_001253783.1 | 35.4% | 31.7% | (many diffs) |
| 37 | mouse | 23854 | Def8 | differentially expressed in... | XM_006530975.2 | 35.4% | 31.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 657
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atatgatgag aagctggccc gtttccggca ggcccacctc aaccccttca 121 acaagcagtc tgggccgaga cagcatgagc agggccctgg ggaggaggtc ccggacgtca 181 ctcctgaaga ggccctgcct gagctgcccc ctggggagcc ggaattccgc tgccctgaac 241 gcgtgatgga tctcggcctg tctgaggacc acttctcccg ccctgtgggt ctgttcctgg 301 ccTCTGACGT CCAGCAGCTG CGGCAGGCGA TCGAGGAGTG CAAGCAGGTG ATTCTGGAGC 361 TGCCCGAGCA GTCGGAGAAG CAGAAGGATG CCGTGGTGCG ACTCATCCAC CTCCGGCTGA 421 AGCTCCAGGA GCTGAAGGAC CCCAATGAGG ATGAGCCAAA CATCCGAGTG CTCCTTGAGC 481 ACCGCTTTTA CAAGGAGAAG AGCAAGAGCG TCAAGCAGAC CTGTGACAAG TGTAACACCA 541 TCATCTGGGG GCTCATTCAG ACCTGGTACA CCTGCACAGG TGGGCCGAGA CCCAGACGAG 601 GAGTGAGGAA TGAGAGAGAC CAAAGTTCCT GCCTCCGCTG GGCTCACATT CAGATGTGCC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GACACGAACC GTACTAAAGG ATGGATACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt