Transcript: Human XM_017023503.2

PREDICTED: Homo sapiens myocardin related transcription factor B (MRTFB), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MRTFB (57496)
Length:
11984
CDS:
3461..6649

Additional Resources:

NCBI RefSeq record:
XM_017023503.2
NBCI Gene record:
MRTFB (57496)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017023503.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232301 GACACTTTCACCGAGATTATG pLKO_005 4919 CDS 100% 13.200 18.480 N MRTFB n/a
2 TRCN0000232303 ACGTTAGGATTCTGGTAATTA pLKO_005 6974 3UTR 100% 15.000 12.000 N MRTFB n/a
3 TRCN0000015397 GCCAGCTTAGAGAACCAACTA pLKO.1 6275 CDS 100% 4.950 3.960 N MRTFB n/a
4 TRCN0000369296 CAAAGACGACTCATCTATTTC pLKO_005 6882 3UTR 100% 13.200 9.240 N MRTFB n/a
5 TRCN0000015393 CCAGTGTTAAAGAAGCAATTA pLKO.1 3840 CDS 100% 13.200 9.240 N MRTFB n/a
6 TRCN0000369298 GGTCACTTTAAGACCCTAAAT pLKO_005 7012 3UTR 100% 13.200 9.240 N MRTFB n/a
7 TRCN0000232299 GTGTTAAAGAAGCAATTATAG pLKO_005 3843 CDS 100% 13.200 9.240 N MRTFB n/a
8 TRCN0000369370 TCTAGCCTGGATGACTTAAAG pLKO_005 4541 CDS 100% 13.200 9.240 N MRTFB n/a
9 TRCN0000232302 TGTCGTCCAGCACTCTCTATT pLKO_005 5944 CDS 100% 13.200 9.240 N MRTFB n/a
10 TRCN0000369299 TTTACCCTCAGCCAATGAAAT pLKO_005 6316 CDS 100% 13.200 9.240 N MRTFB n/a
11 TRCN0000232300 GATCCCAAACCACGGGTAAAG pLKO_005 4232 CDS 100% 10.800 7.560 N MRTFB n/a
12 TRCN0000369368 GGACCTCATTGAGCGCCTAAA pLKO_005 4621 CDS 100% 10.800 7.560 N MRTFB n/a
13 TRCN0000015394 GCAGACACTTTCACCGAGATT pLKO.1 4916 CDS 100% 4.950 3.465 N MRTFB n/a
14 TRCN0000280403 GCAGACACTTTCACCGAGATT pLKO_005 4916 CDS 100% 4.950 3.465 N MRTFB n/a
15 TRCN0000015396 GCCGGTTACAACACTACACAA pLKO.1 4744 CDS 100% 4.950 3.465 N MRTFB n/a
16 TRCN0000015395 CCTCATTAAGAGTGGAGAGAT pLKO.1 6097 CDS 100% 4.950 2.970 N MRTFB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017023503.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12353 pDONR223 100% 32.3% 29.2% None (many diffs) n/a
2 ccsbBroad304_12353 pLX_304 0% 32.3% 29.2% V5 (many diffs) n/a
3 TRCN0000467244 CGATCCCTGGTATGATTTCTCTGG pLX_317 31.4% 32.3% 29.2% V5 (many diffs) n/a
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