Transcript: Human XM_017027710.2

PREDICTED: Homo sapiens adenosylhomocysteinase (AHCY), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AHCY (191)
Length:
2136
CDS:
450..1370

Additional Resources:

NCBI RefSeq record:
XM_017027710.2
NBCI Gene record:
AHCY (191)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027710.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000257396 GAGCTAATGGCACCAACTTTG pLKO_005 1435 3UTR 100% 10.800 15.120 N AHCY n/a
2 TRCN0000218387 ATGCCATTGTGTGTAACATTG pLKO_005 949 CDS 100% 10.800 7.560 N AHCY n/a
3 TRCN0000229329 CCGTCACCAAGAGCAAGTTTG pLKO_005 619 CDS 100% 10.800 7.560 N AHCY n/a
4 TRCN0000229330 GTCAGGAGGGCAACATCTTTG pLKO_005 868 CDS 100% 10.800 7.560 N AHCY n/a
5 TRCN0000229328 TCAAGGTGCCTGCCATCAATG pLKO_005 589 CDS 100% 10.800 7.560 N AHCY n/a
6 TRCN0000050020 CGGGCCACAGATGTGATGATT pLKO.1 684 CDS 100% 5.625 3.938 N AHCY n/a
7 TRCN0000050022 CACAGGCTGTATTGACATCAT pLKO.1 896 CDS 100% 4.950 3.465 N AHCY n/a
8 TRCN0000050021 CCACAACCTCTACAAGATGAT pLKO.1 554 CDS 100% 4.950 3.465 N AHCY n/a
9 TRCN0000050018 GTGTGTAACATTGGACACTTT pLKO.1 957 CDS 100% 4.950 3.465 N AHCY n/a
10 TRCN0000050019 GATGAGTAACTCCTTCACCAA pLKO.1 1142 CDS 100% 2.640 1.848 N AHCY n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027710.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13810 pDONR223 100% 99.8% 99.6% None 908A>C n/a
2 ccsbBroad304_13810 pLX_304 0% 99.8% 99.6% V5 908A>C n/a
3 TRCN0000476516 GACTAAACGACTGCCACTCTTAGA pLX_317 48% 99.8% 99.6% V5 908A>C n/a
4 ccsbBroadEn_00042 pDONR223 100% 70.8% 70.8% None 0_1ins378 n/a
5 ccsbBroad304_00042 pLX_304 0% 70.8% 70.8% V5 0_1ins378 n/a
6 TRCN0000480477 AGCAATATTAAGGTTTTTTGGCGC pLX_317 34.3% 70.8% 70.8% V5 0_1ins378 n/a
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