Transcript: Mouse XM_017314157.1

PREDICTED: Mus musculus ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b), transcript variant X23, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Anks1b (77531)
Length:
2967
CDS:
131..1420

Additional Resources:

NCBI RefSeq record:
XM_017314157.1
NBCI Gene record:
Anks1b (77531)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017314157.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000248243 ATTTATCGATGCGGCAAATAA pLKO_005 982 CDS 100% 15.000 21.000 N Anks1b n/a
2 TRCN0000248245 CCGAACATGAAATTCGTAATA pLKO_005 1014 CDS 100% 13.200 9.240 N Anks1b n/a
3 TRCN0000234649 CTCTCAACATTTGCCTATATC pLKO_005 1064 CDS 100% 13.200 9.240 N ANKS1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017314157.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12309 pDONR223 100% 85.5% 90.7% None (many diffs) n/a
2 ccsbBroad304_12309 pLX_304 0% 85.5% 90.7% V5 (many diffs) n/a
3 TRCN0000474562 TCACTGTACAGTACAGTCAGCCCA pLX_317 16.7% 85.5% 90.7% V5 (many diffs) n/a
4 ccsbBroadEn_15926 pDONR223 0% 76.2% 78% None (many diffs) n/a
5 ccsbBroad304_15926 pLX_304 0% 76.2% 78% V5 (many diffs) n/a
6 TRCN0000481009 CTCTTATTGAGGCACTTGCCAGAA pLX_317 37.9% 76.2% 78% V5 (many diffs) n/a
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