Transcript: Mouse XM_017315005.1

PREDICTED: Mus musculus activin A receptor, type 1 (Acvr1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Acvr1 (11477)
Length:
3159
CDS:
529..2058

Additional Resources:

NCBI RefSeq record:
XM_017315005.1
NBCI Gene record:
Acvr1 (11477)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017315005.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000321758 CTTATAAGAGGGTCGATATTT pLKO_005 1721 CDS 100% 15.000 21.000 N Acvr1 n/a
2 TRCN0000321821 TCGATAATTCCCTAGACAAAT pLKO_005 2021 CDS 100% 13.200 18.480 N Acvr1 n/a
3 TRCN0000321754 TGGTTCTCAGACCCGACATTA pLKO_005 1909 CDS 100% 13.200 18.480 N Acvr1 n/a
4 TRCN0000378240 TGGTTCAGGGAGACGGAATTG pLKO_005 1261 CDS 100% 10.800 8.640 N Acvr1 n/a
5 TRCN0000022521 AGGAAGTTTAAGAGACGCAAT pLKO.1 967 CDS 100% 4.050 3.240 N Acvr1 n/a
6 TRCN0000361058 ACTTGCACTGTGGCCCTTAAT pLKO_005 2509 3UTR 100% 13.200 9.240 N Acvr1 n/a
7 TRCN0000361057 AGTTGCTCTCAGGAAGTTTAA pLKO_005 957 CDS 100% 13.200 9.240 N Acvr1 n/a
8 TRCN0000022519 CATCACACATTACCATGAAAT pLKO.1 1371 CDS 100% 13.200 9.240 N Acvr1 n/a
9 TRCN0000321822 TTGTCCAGCTGGGACCTAATG pLKO_005 2098 3UTR 100% 10.800 7.560 N Acvr1 n/a
10 TRCN0000022520 GCAATGGTATAGTGGAAGATT pLKO.1 1787 CDS 100% 5.625 3.938 N Acvr1 n/a
11 TRCN0000022522 CAGAGCACAAACCAGCTTGAT pLKO.1 1615 CDS 100% 4.950 3.465 N Acvr1 n/a
12 TRCN0000022523 GACATTAACTTCTCTGGCGAA pLKO.1 1923 CDS 100% 2.160 1.512 N Acvr1 n/a
13 TRCN0000199872 GCCTGACTGGTTGTCAGAATG pLKO.1 2122 3UTR 100% 1.080 0.756 N ACVR1 n/a
14 TRCN0000315052 GCCTGACTGGTTGTCAGAATG pLKO_005 2122 3UTR 100% 1.080 0.756 N ACVR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017315005.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05767 pDONR223 100% 90.7% 98.4% None (many diffs) n/a
2 ccsbBroad304_05767 pLX_304 0% 90.7% 98.4% V5 (many diffs) n/a
3 TRCN0000469327 ACCAAAGGCGGGTTTGGTAGCTTC pLX_317 27.6% 90.7% 98.4% V5 (many diffs) n/a
4 ccsbBroadEn_14527 pDONR223 0% 90.7% 98.4% None (many diffs) n/a
5 ccsbBroad304_14527 pLX_304 0% 90.7% 98.4% V5 (many diffs) n/a
6 TRCN0000474710 GATATTCCTACCTCCATACTATTG pLX_317 30.3% 90.7% 98.4% V5 (many diffs) n/a
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