Construct: ORF TRCN0000471857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012812.1_s317c1
- Derived from:
- ccsbBroadEn_12488
- DNA Barcode:
- TTCTCTGACAGATAGAGAATCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCED1A (64773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471857
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260806.4 | 81.4% | 80.3% | (many diffs) |
| 2 | human | 64773 | PCED1A | PC-esterase domain containi... | NM_001271168.2 | 77.3% | 77.1% | 605C>T;635_907del |
| 3 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260805.3 | 77.3% | 77.1% | 605C>T;635_907del |
| 4 | human | 64773 | PCED1A | PC-esterase domain containi... | NM_022760.6 | 68.6% | 68.5% | 203_355del;758C>T;788_1060del |
| 5 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260804.2 | 68.6% | 68.5% | 203_355del;758C>T;788_1060del |
| 6 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_006499706.3 | 71.7% | 70.7% | (many diffs) |
| 7 | mouse | 319513 | Pced1a | PC-esterase domain containi... | NM_001114541.1 | 59.6% | 59% | (many diffs) |
| 8 | mouse | 319513 | Pced1a | PC-esterase domain containi... | NM_178762.4 | 59.6% | 59% | (many diffs) |
| 9 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_017318973.1 | 59.6% | 59% | (many diffs) |
| 10 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_006499709.3 | 56.8% | 54.9% | (many diffs) |
| 11 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_017318975.1 | 44.5% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cttctgtctg tcgagcgagg agccgcgccg cccgctgcga agcgacatgg 121 tccacttcca ggcctcggaa gtccagcagc tgctacacaa caagttcgtg gtcatcttgg 181 gggactccat tcagagggct gtgtacaagg acctggtgct cttgctccag aaagactcac 241 tgctcacagc tgcccagctg aaagccaagt accttgagga tgttctggaa gagctgacat 301 atggacctgc cccggacctg gtgatcatca actcctgcct ctgggatctc tccagatatg 361 gtcgctgctc aatggagagc taccgggaga acctggagcg ggtgtttgtg cgcatggacc 421 aagtattgcc agactcctgc ctgctggtgt ggaacatggc gatgcccctc ggggaacgta 481 tcactggggg tttcctcctg ccagagctcc agcccctggc aggctccctg cggcgggatg 541 tggttgaagg gaacttctac agtgctacgc tggccgggga ccactgcttt gatgtcctag 601 accTCCACTT TCACTTCCGG CATGCAGTAC AGCACCGTCA TCGGGATGGT GTCCACTGGG 661 ACCAGCATGT ACACCGCCAC CTCTCACACC TGCTTCTGAC CCATGAATTC TTCAACTATA 721 ATCCAGTGGA GGACTTCTCG ATGCCACCCC ACTTAGGATG TGGCCCTGGA GTGAACTTTG 781 TGCCTGGCCC TCTGCCACCT CCAATCCCTG GCCCTAATCC CCATGGTCAG CACTGGGGCC 841 CAGTGGTCCA CCGGGGGATG CCACGCTATG TTCCTAACAG CCCCTACCAT GTGCGGAGAA 901 TGGGGGGGCC CTGCAGGCAG CGGCTCAGAC ACTCAGAGAG ACTGATCCAC ACATACAAAC 961 TGGACAGACG GCCTCCTGCC CATTCGGGGA CATGGCCTGG GCACCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATTC TCTGACAGAT AGAGAATCCT AACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t