Construct: ORF TRCN0000471857

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012812.1_s317c1
Derived from:
ccsbBroadEn_12488
DNA Barcode:
TTCTCTGACAGATAGAGAATCCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCED1A (64773)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471857
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64773 PCED1A PC-esterase domain containi... XM_005260806.4 81.4% 80.3% (many diffs)
2 human 64773 PCED1A PC-esterase domain containi... NM_001271168.2 77.3% 77.1% 605C>T;635_907del
3 human 64773 PCED1A PC-esterase domain containi... XM_005260805.3 77.3% 77.1% 605C>T;635_907del
4 human 64773 PCED1A PC-esterase domain containi... NM_022760.6 68.6% 68.5% 203_355del;758C>T;788_1060del
5 human 64773 PCED1A PC-esterase domain containi... XM_005260804.2 68.6% 68.5% 203_355del;758C>T;788_1060del
6 mouse 319513 Pced1a PC-esterase domain containi... XM_006499706.3 71.7% 70.7% (many diffs)
7 mouse 319513 Pced1a PC-esterase domain containi... NM_001114541.1 59.6% 59% (many diffs)
8 mouse 319513 Pced1a PC-esterase domain containi... NM_178762.4 59.6% 59% (many diffs)
9 mouse 319513 Pced1a PC-esterase domain containi... XM_017318973.1 59.6% 59% (many diffs)
10 mouse 319513 Pced1a PC-esterase domain containi... XM_006499709.3 56.8% 54.9% (many diffs)
11 mouse 319513 Pced1a PC-esterase domain containi... XM_017318975.1 44.5% 44.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1002
ORF length:
936
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt cttctgtctg tcgagcgagg agccgcgccg cccgctgcga agcgacatgg
121tccacttcca ggcctcggaa gtccagcagc tgctacacaa caagttcgtg gtcatcttgg
181gggactccat tcagagggct gtgtacaagg acctggtgct cttgctccag aaagactcac
241tgctcacagc tgcccagctg aaagccaagt accttgagga tgttctggaa gagctgacat
301atggacctgc cccggacctg gtgatcatca actcctgcct ctgggatctc tccagatatg
361gtcgctgctc aatggagagc taccgggaga acctggagcg ggtgtttgtg cgcatggacc
421aagtattgcc agactcctgc ctgctggtgt ggaacatggc gatgcccctc ggggaacgta
481tcactggggg tttcctcctg ccagagctcc agcccctggc aggctccctg cggcgggatg
541tggttgaagg gaacttctac agtgctacgc tggccgggga ccactgcttt gatgtcctag
601accTCCACTT TCACTTCCGG CATGCAGTAC AGCACCGTCA TCGGGATGGT GTCCACTGGG
661ACCAGCATGT ACACCGCCAC CTCTCACACC TGCTTCTGAC CCATGAATTC TTCAACTATA
721ATCCAGTGGA GGACTTCTCG ATGCCACCCC ACTTAGGATG TGGCCCTGGA GTGAACTTTG
781TGCCTGGCCC TCTGCCACCT CCAATCCCTG GCCCTAATCC CCATGGTCAG CACTGGGGCC
841CAGTGGTCCA CCGGGGGATG CCACGCTATG TTCCTAACAG CCCCTACCAT GTGCGGAGAA
901TGGGGGGGCC CTGCAGGCAG CGGCTCAGAC ACTCAGAGAG ACTGATCCAC ACATACAAAC
961TGGACAGACG GCCTCCTGCC CATTCGGGGA CATGGCCTGG GCACCCAACT TTCTTGTACA
1021AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1081AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1141AGGACGATTC TCTGACAGAT AGAGAATCCT AACGCGTTAA GTCgacaatc aacctctgga
1201ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)