Transcript: Mouse XM_017320040.1

PREDICTED: Mus musculus phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pik3r3 (18710)
Length:
4502
CDS:
131..1516

Additional Resources:

NCBI RefSeq record:
XM_017320040.1
NBCI Gene record:
Pik3r3 (18710)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320040.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361422 GAGCGAATTATGATGAATTAT pLKO_005 890 CDS 100% 15.000 21.000 N Pik3r3 n/a
2 TRCN0000361423 TATTCGACAGAACTGATATTT pLKO_005 194 CDS 100% 15.000 21.000 N Pik3r3 n/a
3 TRCN0000025089 CGAGCGAATTATGATGAATTA pLKO.1 889 CDS 100% 13.200 18.480 N Pik3r3 n/a
4 TRCN0000025091 CCTGCCGCATTATGATGAGAA pLKO.1 1177 CDS 100% 4.950 6.930 N Pik3r3 n/a
5 TRCN0000025090 GCCCTATTCGACAGAACTGAT pLKO.1 190 CDS 100% 4.950 6.930 N Pik3r3 n/a
6 TRCN0000025092 GCAAGAAAGGATGTTACGCTT pLKO.1 1287 CDS 100% 2.640 3.696 N Pik3r3 n/a
7 TRCN0000361352 CTTCTGTGGTGGAGCTTATTA pLKO_005 522 CDS 100% 15.000 10.500 N Pik3r3 n/a
8 TRCN0000033291 CCTATTCGACAGAACTGATAT pLKO.1 192 CDS 100% 13.200 9.240 N PIK3R3 n/a
9 TRCN0000361421 GATGAGAACCTGCCGCATTAT pLKO_005 1169 CDS 100% 13.200 9.240 N Pik3r3 n/a
10 TRCN0000033292 CATTCTTAATTCGTGAGAGTA pLKO.1 1266 CDS 100% 4.950 3.465 N PIK3R3 n/a
11 TRCN0000025093 CCAACAGGATCAGTTGGTAAA pLKO.1 619 CDS 100% 1.080 0.756 N Pik3r3 n/a
12 TRCN0000033289 GCTCAGTACAATCCCAAACTT pLKO.1 566 CDS 100% 5.625 3.938 N PIK3R3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320040.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07249 pDONR223 100% 91.7% 95.6% None (many diffs) n/a
2 ccsbBroad304_07249 pLX_304 0% 91.7% 95.6% V5 (many diffs) n/a
3 TRCN0000478621 TAGACCCCGAATACATCTAAATCC pLX_317 24.3% 91.7% 95.6% V5 (many diffs) n/a
4 ccsbBroadEn_14899 pDONR223 0% 91.7% 95.6% None (many diffs) n/a
5 ccsbBroad304_14899 pLX_304 0% 91.7% 95.6% V5 (many diffs) n/a
6 TRCN0000480772 CTCCATTTCTTCTAAATTGTACCA pLX_317 27.3% 91.7% 95.6% V5 (many diffs) n/a
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