Transcript: Mouse XM_017320714.1

PREDICTED: Mus musculus postmeiotic segregation increased 2 (S. cerevisiae) (Pms2), transcript variant X16, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pms2 (18861)
Length:
2239
CDS:
19..1356

Additional Resources:

NCBI RefSeq record:
XM_017320714.1
NBCI Gene record:
Pms2 (18861)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320714.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000240635 TCGACTGGTGTTTGACCATAA pLKO_005 555 CDS 100% 10.800 15.120 N Pms2 n/a
2 TRCN0000240634 ACTAAGTGATGTCACTATATC pLKO_005 504 CDS 100% 13.200 9.240 N Pms2 n/a
3 TRCN0000240633 ACCGGCATCAGTACCCATTTG pLKO_005 1097 CDS 100% 10.800 7.560 N Pms2 n/a
4 TRCN0000180814 GCGGATGAGAAGTACAACTTT pLKO.1 1401 3UTR 100% 5.625 3.938 N Pms2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320714.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14772 pDONR223 92.2% 37% 37.5% None (many diffs) n/a
2 ccsbBroad304_14772 pLX_304 0% 37% 37.5% V5 (many diffs) n/a
3 TRCN0000480058 GCTATCTCATTGCACGAGGCGAAG pLX_317 12.6% 37% 37.5% V5 (many diffs) n/a
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