Construct: ORF ccsbBroad304_14772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013128.1_s304c1, BRDN0000462181
- Derived from:
- ccsbBroadEn_14772
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMS2 (5395)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14772
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_000535.7 | 99.8% | 99.7% | 780C>G;1621A>G;1866G>A |
| 2 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322014.2 | 98.6% | 98.5% | (many diffs) |
| 3 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322006.2 | 93.8% | 93.7% | (many diffs) |
| 4 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322015.2 | 87.7% | 86.2% | (many diffs) |
| 5 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322007.1 | 87.5% | 86.5% | (many diffs) |
| 6 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322008.2 | 87.5% | 86.5% | (many diffs) |
| 7 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322003.2 | 84.2% | 84.1% | (many diffs) |
| 8 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322004.2 | 84.2% | 84.1% | (many diffs) |
| 9 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322005.2 | 84.2% | 84.1% | (many diffs) |
| 10 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322009.2 | 83.1% | 83% | (many diffs) |
| 11 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322010.2 | 78.1% | 78% | (many diffs) |
| 12 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_024446800.1 | 78.1% | 78% | (many diffs) |
| 13 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322013.2 | 77.7% | 77.6% | (many diffs) |
| 14 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322011.2 | 63.8% | 63.6% | 0_1ins933;688A>G;933G>A |
| 15 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322012.2 | 63.8% | 63.6% | 0_1ins933;688A>G;933G>A |
| 16 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_006715744.4 | 63.8% | 63.6% | 0_1ins933;688A>G;933G>A |
| 17 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_017012342.2 | 63.8% | 63.6% | 0_1ins933;688A>G;933G>A |
| 18 | human | 441194 | PMS2CL | PMS2 C-terminal like pseudo... | NR_002217.1 | 53.4% | (many diffs) | |
| 19 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NR_136154.1 | 49.2% | (many diffs) | |
| 20 | human | 5382 | PMS2P4 | PMS1 homolog 2, mismatch re... | NR_046299.1 | 21.8% | (many diffs) | |
| 21 | human | 100101440 | PMS2P7 | PMS1 homolog 2, mismatch re... | NR_130940.1 | 20.3% | (many diffs) | |
| 22 | human | 5380 | PMS2P2 | PMS1 homolog 2, mismatch re... | NR_003614.3 | 19.8% | (many diffs) | |
| 23 | human | 5382 | PMS2P4 | PMS1 homolog 2, mismatch re... | NR_022007.2 | 18.8% | (many diffs) | |
| 24 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784638.1 | 44.4% | (many diffs) | |
| 25 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784639.1 | 44% | (many diffs) | |
| 26 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XM_017320714.1 | 37% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2655
- ORF length:
- 2586
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcgagct gagagctcga gtacagaacc tgctaaggcc atcaaaccta 121 ttgatcggaa gtcagtccat cagatttgct ctgggcaggt ggtactgagt ctaagcactg 181 cggtaaagga gttagtagaa aacagtctgg atgctggtgc cactaatatt gatctaaagc 241 ttaaggacta tggagtggat cttattgaag tttcagacaa tggatgtggg gtagaagaag 301 aaaacttcga aggcttaact ctgaaacatc acacatctaa gattcaagag tttgccgacc 361 taactcaggt tgaaactttt ggctttcggg gggaagctct gagctcactt tgtgcactga 421 gcgatgtcac catttctacc tgccacgcat cggcgaaggt tggaactcga ctgatgtttg 481 atcacaatgg gaaaattatc cagaaaaccc cctacccccg ccccagaggg accacagtca 541 gcgtgcagca gttattttcc acactacctg tgcgccataa ggaatttcaa aggaatatta 601 agaaggagta tgccaaaatg gtccaggtct tacatgcata ctgtatcatt tcagcaggca 661 tccgtgtaag ttgcaccaat cagcttggac aaggaaaacg acagcctgtg gtatgcacag 721 gtggaagccc cagcataaag gaaaatatcg gctctgtgtt tgggcagaag cagttgcaaa 781 gcctcattcc ttttgttcag ctgcccccta gtgactccgt gtgtgaagag tacggtttga 841 gctgttcgga tgctctgcat aatctttttt acatctcagg tttcatttca caatgcacgc 901 atggagttgg aaggagttca acagacagac agtttttctt tatcaaccgg cggccttgtg 961 acccagcaaa ggtctgcaga ctcgtgaatg aggtctacca catgtataat cgacaccagt 1021 atccatttgt tgttcttaac atttctgttg attcagaatg cgttgatatc aatgttactc 1081 cagataaaag gcaaattttg ctacaagagg aaaagctttt gttggcagtt ttaaagacct 1141 ctttgatagg aatgtttgat agtgatgtca acaagctaaa tgtcagtcag cagccactgc 1201 tggatgttga aggtaactta ataaaaatgc atgcagcgga tttggaaaag cccatggtag 1261 aaaagcagga tcaatcccct tcattaagga ctggagaaga aaaaaaagac gtgtccattt 1321 ccagactgcg agaggccttt tctcttcgtc acacaacaga gaacaagcct cacagcccaa 1381 agactccaga accaagaagg agccctctag gacagaaaag gggtatgctg tcttctagca 1441 cttcaggtgc catctctgac aaaggcgtcc tgagacctca gaaagaggca gtgagttcca 1501 gtcacggacc cagtgaccct acggacagag cggaggtgga gaaggactcg gggcacggca 1561 gcacttccgt ggattctgag gggttcagca tcccagacac gggcagtcac tgcagcagcg 1621 agtatgcggc cagctcccca ggggacaggg gctcgcagga acatgtggac tctcaggaga 1681 aagcgcctga aactgacgac tctttttcag atgtggactg ccattcaaac caggaagata 1741 ccggatgtaa atttcgagtt ttgcctcagc caactaatct cgcaacccca aacacaaagc 1801 gttttaaaaa agaagaaatt ctttccagtt ctgacatttg tcaaaagtta gtaaatactc 1861 aggacatgtc agcctctcag gttgatgtag ctgtgaaaat taataagaaa gttgtgcccc 1921 tggacttttc tataagttct ttagctaaac gaataaagca gttacatcat gaagcacagc 1981 aaagtgaagg ggaacagaat tacaggaagt ttagggcaaa gatttgtcct ggagaaaatc 2041 aagcagccga agatgaacta agaaaagaga taagtaaaac gatgtttgca gaaatggaaa 2101 tcattggtca gtttaacctg ggatttataa taaccaaact gaatgaggat atcttcatag 2161 tggaccagca tgccacggac gagaagtata acttcgagat gctgcagcag cacaccgtgc 2221 tccaggggca gaggctcata gcacctcaga ctctcaactt aactgctgtt aatgaagctg 2281 ttctgataga aaatctggaa atatttagaa agaatggctt tgattttgtt atcgatgaaa 2341 atgctccagt cactgaaagg gctaaactga tttccttgcc aactagtaaa aactggacct 2401 tcggacccca ggacgtcgat gaactgatct tcatgctgag cgacagccct ggggtcatgt 2461 gccggccttc ccgagtcaag cagatgtttg cctccagagc ctgccggaag tcggtgatga 2521 ttgggactgc tcttaacaca agcgagatga agaaactgat cacccacatg ggggagatgg 2581 accacccctg gaactgtccc catggaaggc caaccatgag acacatcgcc aacctgggtg 2641 tcatttctca gaacttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 2701 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2761 cgacaatcaa cctctggatt a