Transcript: Mouse XM_017321114.1

PREDICTED: Mus musculus zinc finger protein 655 (Zfp655), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zfp655 (72611)
Length:
4085
CDS:
113..1738

Additional Resources:

NCBI RefSeq record:
XM_017321114.1
NBCI Gene record:
Zfp655 (72611)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017321114.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000427400 TGGTGAACAAGAACCAATAAA pLKO_005 721 CDS 100% 15.000 12.000 N Zfp655 n/a
2 TRCN0000095628 CACACCTCATACTTAGTTGAA pLKO.1 1226 CDS 100% 4.950 3.960 N Zfp655 n/a
3 TRCN0000095626 CCTTTGGAGAACCAGTCTGAA pLKO.1 167 CDS 100% 4.950 3.960 N Zfp655 n/a
4 TRCN0000434470 GACCACTGATGACAGTAAATA pLKO_005 670 CDS 100% 15.000 10.500 N Zfp655 n/a
5 TRCN0000438214 ATGTGGGCTGGCCTATGTTAA pLKO_005 1291 CDS 100% 13.200 9.240 N Zfp655 n/a
6 TRCN0000435150 TATACCCAGAAATAGTCTATA pLKO_005 2156 3UTR 100% 13.200 9.240 N Zfp655 n/a
7 TRCN0000095625 GCACACCTCATCCAACATGAA pLKO.1 1634 CDS 100% 4.950 3.465 N Zfp655 n/a
8 TRCN0000095627 GCTGTTGATTCCTAAGCGGAA pLKO.1 379 CDS 100% 2.160 1.512 N Zfp655 n/a
9 TRCN0000095624 CCGCTGAATGTGATGAACATT pLKO.1 1842 3UTR 100% 0.563 0.394 N Zfp655 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017321114.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08908 pDONR223 100% 76.4% 72.6% None (many diffs) n/a
2 ccsbBroad304_08908 pLX_304 0% 76.4% 72.6% V5 (many diffs) n/a
3 TRCN0000467232 CCATCTTTAAACGACTAACACCTT pLX_317 29.8% 76.4% 72.6% V5 (many diffs) n/a
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