Construct: ORF TRCN0000467232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000767.1_s317c1
- Derived from:
- ccsbBroadEn_08908
- DNA Barcode:
- CCATCTTTAAACGACTAACACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF655 (79027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001009960.1 | 99.9% | 99.7% | 156G>T |
| 2 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001363334.1 | 99.9% | 99.7% | 156G>T |
| 3 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_138494.3 | 99.9% | 99.7% | 156G>T |
| 4 | human | 79027 | ZNF655 | zinc finger protein 655 | XM_017012607.2 | 99.9% | 99.7% | 156G>T |
| 5 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001083956.1 | 93.2% | 93.1% | 136_240del;261G>T |
| 6 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001085368.1 | 93.2% | 93.1% | 136_240del;261G>T |
| 7 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001363332.1 | 93.2% | 93.1% | 136_240del;261G>T |
| 8 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001363333.1 | 93.2% | 93.1% | 136_240del;261G>T |
| 9 | human | 100131257 | LOC100131257 | zinc finger protein 655 pse... | NR_034022.1 | 6.1% | (many diffs) | |
| 10 | mouse | 72611 | Zfp655 | zinc finger protein 655 | NM_028298.3 | 76.4% | 72.6% | (many diffs) |
| 11 | mouse | 72611 | Zfp655 | zinc finger protein 655 | XM_017321114.1 | 76.4% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaaatacca gcccaggaag cagcagggtc accaagggtc cagtttcagt 121 ctttggagac ccagtctgag tgtctgtccc cagagcctca gtttgtgcag gacaccgaca 181 tggaacaggg actcactggg gatggagaga ccagagaaga taacaagctg ttgattccta 241 agcagaaaat ttcggaagaa gtgcattcat acaaagtgag agtaggaaga ctcaaacacg 301 atattaccca agttcctgag actagagaag tgtataagtc tgaggacaga ttagaaagac 361 ttcaggaaat tctaaggaaa tttctgtacc tggagagaga gtttaggcaa ataacaatca 421 gcaaggaaac cttcaccagt gagaagaaca atgaatgtca tgaacccgaa aaaagcttca 481 gtctggactc tactattgat gcagatcaga gagttcttag aatacagaat accgatgaca 541 atgataagta tgacatgagc ttcaaccaga attcagcctc tggtaaacat gaacacttaa 601 atctaacaga ggattttcag agtagtgaat gtaaggaaag cttaatggat ctctcccacc 661 ttaataaatg ggagagcatc cctaacactg agaaatccta taaatgtgat gtatgtggga 721 aaattttcca tcagagctca gcccttacta gacatcagag aatccatact agagagaagc 781 cctacaaatg taaagaatgt gaaaagtctt tcagtcagag ctcaagtctt agtcgacata 841 aaagaataca cactagagaa aaaccttaca aatgtgaagc atctgataaa tcctgtgaag 901 cgtctgataa atcctgtagt ccaagctcag gcataattca gcataagaaa attcacacca 961 gagccaaatc ttacaaatgt agcagttgtg aaagagtctt cagtcgtagt gtccacctta 1021 ctcaacatca gaaaattcac aaagagatgc cctgtaagtg tactgtatgt ggcagtgact 1081 tctgccatac ttcataccta cttGAACATC AGAGGGTCCA TCATGAAGAG AAAGCCTATG 1141 AGTATGATGA ATATGGGTTG GCCTATATTA AACAACAAGG AATTCATTTC AGAGAAAAGC 1201 CCTATACGTG TAGTGAATGT GGAAAAGACT TCAGATTGAA TTCACATCTT ATTCAGCATC 1261 AAAGAATTCA CACAGGAGAG AAAGCACATG AATGTAATGA ATGTGGAAAA GCTTTCAGTC 1321 AAACCTCATG CCTTATTCAG CATCACAAAA TGCATAGGAA AGAGAAATCG TATGAATGTA 1381 ATGAGTATGA GGGCAGTTTC AGTCATAGCT CAGATCTTAT CCTGCAACAA GAAGTCCTCA 1441 CCAGACAGAA AGCCTTTGAT TGTGATGTAT GGGAAAAGAA CTCCAGTCAG AGAGCACATC 1501 TAGTTCAACA TCAGAGCATT CATACCAAAG AGAACTCATG CCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGACCATCT TTAAACGACT AACACCTTAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt