Transcript: Human XM_024450834.1

PREDICTED: Homo sapiens ArfGAP with dual PH domains 2 (ADAP2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ADAP2 (55803)
Length:
2688
CDS:
74..1159

Additional Resources:

NCBI RefSeq record:
XM_024450834.1
NBCI Gene record:
ADAP2 (55803)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024450834.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145603 CAATGGATTCGAGCTAAGTAT pLKO.1 422 CDS 100% 5.625 7.875 N ADAP2 n/a
2 TRCN0000292161 CAATGGATTCGAGCTAAGTAT pLKO_005 422 CDS 100% 5.625 7.875 N ADAP2 n/a
3 TRCN0000141491 CGCAGAAATACACTGGATGTT pLKO.1 1815 3UTR 100% 4.950 6.930 N ADAP2 n/a
4 TRCN0000292162 TGAAAGATAAAGTAGTCAATG pLKO_005 1617 3UTR 100% 10.800 7.560 N ADAP2 n/a
5 TRCN0000106217 GCCTGGTCTTAAAGGAACAAT pLKO.1 405 CDS 100% 5.625 3.938 N Adap2 n/a
6 TRCN0000141961 GCCTGGTCTTAAAGGAACAAT pLKO.1 405 CDS 100% 5.625 3.938 N ADAP2 n/a
7 TRCN0000292159 GCCTGGTCTTAAAGGAACAAT pLKO_005 405 CDS 100% 5.625 3.938 N ADAP2 n/a
8 TRCN0000323877 GCCTGGTCTTAAAGGAACAAT pLKO_005 405 CDS 100% 5.625 3.938 N Adap2 n/a
9 TRCN0000139150 CAGATCACCTACAGGAGAGAT pLKO.1 686 CDS 100% 4.950 3.465 N ADAP2 n/a
10 TRCN0000141746 GAGGCTGCTCTATTACAAGAA pLKO.1 946 CDS 100% 4.950 3.465 N ADAP2 n/a
11 TRCN0000292160 GAGGCTGCTCTATTACAAGAA pLKO_005 946 CDS 100% 4.950 3.465 N ADAP2 n/a
12 TRCN0000140694 GCATCATGCAAGTGGCATCTT pLKO.1 1483 3UTR 100% 4.950 3.465 N ADAP2 n/a
13 TRCN0000141899 GCCAAAGCAGAAAGAACCTTT pLKO.1 889 CDS 100% 4.950 3.465 N ADAP2 n/a
14 TRCN0000292158 GCCAAAGCAGAAAGAACCTTT pLKO_005 889 CDS 100% 4.950 3.465 N ADAP2 n/a
15 TRCN0000141030 CACATCTGAAATGGAGGCATT pLKO.1 1442 3UTR 100% 4.050 2.835 N ADAP2 n/a
16 TRCN0000142630 CTTCACAAAGGAACAGGGTAA pLKO.1 586 CDS 100% 4.050 2.835 N ADAP2 n/a
17 TRCN0000139232 CCACGCAGAAATACACTGGAT pLKO.1 1812 3UTR 100% 2.640 1.848 N ADAP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024450834.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12276 pDONR223 100% 91.4% 91.4% None 224_241del;821_823delAGC;1050_1051ins78 n/a
2 ccsbBroad304_12276 pLX_304 0% 91.4% 91.4% V5 224_241del;821_823delAGC;1050_1051ins78 n/a
3 TRCN0000478368 GGCCAGGCGATGAATATCAAATCT pLX_317 32.9% 91.4% 91.4% V5 224_241del;821_823delAGC;1050_1051ins78 n/a
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