Construct: ORF TRCN0000478368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016804.2_s317c1
- Derived from:
- ccsbBroadEn_12276
- DNA Barcode:
- GGCCAGGCGATGAATATCAAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAP2 (55803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | NM_001346714.1 | 100% | 100% | |
| 2 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | NM_018404.3 | 99.7% | 99.7% | 803_805delAGC |
| 3 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | XM_024450832.1 | 98.4% | 98.4% | 224_241del |
| 4 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | NM_001346712.1 | 98.1% | 98.1% | 224_241del;821_823delAGC |
| 5 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | XM_024450833.1 | 93.2% | 93% | 224_241del;760_761ins60 |
| 6 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | XM_024450831.1 | 93.1% | 92.3% | (many diffs) |
| 7 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | NM_001346716.1 | 92.9% | 92.9% | 803_805delAGC;1032_1033ins78 |
| 8 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | XM_024450834.1 | 91.4% | 91.4% | 224_241del;821_823delAGC;1050_1051ins78 |
| 9 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | XM_024450835.1 | 67.7% | 67.7% | 0_1ins366;437_439delAGC |
| 10 | human | 55803 | ADAP2 | ArfGAP with dual PH domains 2 | NR_144488.1 | 38.5% | (many diffs) | |
| 11 | mouse | 216991 | Adap2 | ArfGAP with dual PH domains 2 | NM_172133.1 | 86.3% | 84.5% | (many diffs) |
| 12 | mouse | 216991 | Adap2 | ArfGAP with dual PH domains 2 | XM_006532973.3 | 59.9% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgatcgcgag cgcaacaaga agcggctgct ggagctgctg cgggcgccgg 121 acacaggcaa cgcgcactgc gccgactgcg gggcggcaga tcccgactgg gcctcttaca 181 agctggggat cttcatctgt ctcaactgct gcggcgtcca ccgtaacttc cctgacatca 241 gcagagttaa atctgtgcga cttgacttct gggacgacag tattgtggag tttatgatcc 301 acaatggaaa cctccgtgtg aaggccaagt tcgaagccag agtcccagct ttctactaca 361 tcccccaggc caacgactgc ctggtcttaa aggaacaatg gattcgagct aagtatgaga 421 gacgggaatt tatggctgat ggggaaacca tctcgctccc aggtaaccga gaaggattcc 481 tgtggaagcg aggaagggac aactcacagt ttctgagaag gaagtttgta cttctggcaa 541 gagaaggcct cctgaagtac ttcacaaagg aacagggtaa aagccccaaa gctgtcatca 601 gcattaagga cttgaatgcc accttccaga cagagaagat agggcacccc catgggctgc 661 agatcaccta caggagagat ggccacacca ggaacctgtt tgtgtatcat gaaagtggga 721 aggagatagt ggactggttc aatgccctcc gtgcagcccg tctgcagtac ctaaaaatgg 781 cctttcctga actcccagag tctgagctcg tgccattcct caccaggaac taccTCAAAC 841 AAGGCTTCAT GGAAAAGACT GGGCCAAAGA AAGAACCTTT CAAGAAAAGG TGGTTCGCCC 901 TGGATTGCCA TGAGCGGAGG CTGCTCTATT ACAAGAACCC ACTGGATGCC TTCGAGCAGG 961 GCCAGGTTTT TCTTGGGAAC AAGGAGCAGG GATATGAAGC CTACGAAGAC CTGCCCAAGG 1021 GCATCCGAGG AAATCGCTGG AAAGCCGGAC TCACCATTGT CACCCCAGAG CGGAGATTTG 1081 TCCTCACTTG CCCCAGTGAG AAGGAACAGC AGGAATGGCT GGAAAGTTTG CGGGGTGTCC 1141 TGTCCAGCCC CTTGACGCCC CTCAACCGGC TTACTGCATC AACAGAGAGT GGCCGCAGCA 1201 GCAGGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGCCAGGCG ATGAATATCA AATCTACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt