Transcript: Human XM_024452063.1

PREDICTED: Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
USP25 (29761)
Length:
5183
CDS:
885..3599

Additional Resources:

NCBI RefSeq record:
XM_024452063.1
NBCI Gene record:
USP25 (29761)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452063.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004369 GCGTGAGCTGAGGTATCTATT pLKO.1 887 CDS 100% 13.200 18.480 N USP25 n/a
2 TRCN0000318645 GCGTGAGCTGAGGTATCTATT pLKO_005 887 CDS 100% 13.200 18.480 N USP25 n/a
3 TRCN0000004366 GCACTTCTCCTGTTGACGATA pLKO.1 1615 CDS 100% 4.950 6.930 N USP25 n/a
4 TRCN0000318646 GCACTTCTCCTGTTGACGATA pLKO_005 1615 CDS 100% 4.950 6.930 N USP25 n/a
5 TRCN0000004370 TGGAGGAGTAAGATGAAATAT pLKO.1 4906 3UTR 100% 15.000 10.500 N USP25 n/a
6 TRCN0000004367 GCTGTAGAAGATATGAGAAAT pLKO.1 3372 CDS 100% 13.200 9.240 N USP25 n/a
7 TRCN0000318647 GCTGTAGAAGATATGAGAAAT pLKO_005 3372 CDS 100% 13.200 9.240 N USP25 n/a
8 TRCN0000004368 GCTGTTCCTCATCTGTGCTTA pLKO.1 3122 CDS 100% 4.950 3.465 N USP25 n/a
9 TRCN0000349572 GCTGTTCCTCATCTGTGCTTA pLKO_005 3122 CDS 100% 4.950 3.465 N USP25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452063.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03088 pDONR223 100% 74.1% 74.1% None 0_1ins663;1674_1883del n/a
2 ccsbBroad304_03088 pLX_304 0% 74.1% 74.1% V5 0_1ins663;1674_1883del n/a
3 TRCN0000467162 GTTAGACCGGTCGCTGGTCCGGGT pLX_317 14.4% 74.1% 74.1% V5 0_1ins663;1674_1883del n/a
4 ccsbBroadEn_11902 pDONR223 100% 39% 25.3% None (many diffs) n/a
5 ccsbBroad304_11902 pLX_304 0% 39% 25.3% V5 (many diffs) n/a
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