Construct: ORF TRCN0000467162

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017710.1_s317c1
Derived from:
ccsbBroadEn_03088
DNA Barcode:
GTTAGACCGGTCGCTGGTCCGGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP25 (29761)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467162
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29761 USP25 ubiquitin specific peptidas... NM_013396.6 100% 100%
2 human 29761 USP25 ubiquitin specific peptidas... NM_001283042.3 97% 97% 2337_2432del
3 human 29761 USP25 ubiquitin specific peptidas... NM_001283041.2 93.7% 93.7% 2337_2546del
4 human 29761 USP25 ubiquitin specific peptidas... NM_001352560.2 79% 79% 0_1ins663
5 human 29761 USP25 ubiquitin specific peptidas... XM_011529529.1 77.8% 77% (many diffs)
6 human 29761 USP25 ubiquitin specific peptidas... XM_017028325.1 74.5% 73.5% (many diffs)
7 human 29761 USP25 ubiquitin specific peptidas... XM_017028322.1 74.1% 74.1% 0_1ins663;1674_1883del
8 human 29761 USP25 ubiquitin specific peptidas... XM_017028323.2 74.1% 74.1% 0_1ins663;1674_1883del
9 human 29761 USP25 ubiquitin specific peptidas... XM_024452063.1 74.1% 74.1% 0_1ins663;1674_1883del
10 human 29761 USP25 ubiquitin specific peptidas... XM_024452064.1 70.3% 68.6% 0_1ins715;65_66ins77;1545_1754del
11 human 29761 USP25 ubiquitin specific peptidas... NM_001352561.2 61.7% 61.7% 0_1ins1212
12 human 29761 USP25 ubiquitin specific peptidas... XR_002958593.1 60.6% 1_447del;2439_2459del;3634_5220del
13 human 29761 USP25 ubiquitin specific peptidas... XR_002958592.1 59.5% 1_447del;3043_3153del;3724_5313del
14 human 29761 USP25 ubiquitin specific peptidas... XR_001754834.1 58% 1_447del;2640_2641ins143;3470_5059del
15 human 29761 USP25 ubiquitin specific peptidas... XM_005260949.4 57.8% 57.8% 0_1ins1212;1125_1334del
16 human 29761 USP25 ubiquitin specific peptidas... XM_017028327.1 57.8% 57.8% 0_1ins1212;1125_1334del
17 human 29761 USP25 ubiquitin specific peptidas... XM_011529535.2 53.5% 53.4% 1693_1696delGGTT;1698T>A;1701_1702ins1468
18 mouse 30940 Usp25 ubiquitin specific peptidas... NM_013918.2 87.8% 93.8% (many diffs)
19 mouse 30940 Usp25 ubiquitin specific peptidas... XM_006523056.2 82.3% 88% (many diffs)
20 mouse 30940 Usp25 ubiquitin specific peptidas... XM_006523055.2 81.7% 87.3% (many diffs)
21 mouse 30940 Usp25 ubiquitin specific peptidas... XM_006523057.1 56.2% 59.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3231
ORF length:
3165
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac cgtggagcag aacgtgctgc agcagagcgc ggcgcagaag caccagcaga
121cgtttttgaa tcaactgaga gaaattacgg ggattaatga cacccagata ctacagcaag
181ccttgaagga tagtaatgga aacttggaat tagcagtggc tttccttact gcgaagaatg
241ctaagacccc tcagcaggag gagacaactt actaccaaac agcacttcct ggcaatgata
301gatacatcag tgtgggaagc caagcagata caaatgtgat tgatctcact ggagatgata
361aagatgatct tcagagagca attgccttga gtttggccga atcaaacagg gcattcaggg
421agactggaat aactgatgag gaacaagcca ttagcagagt tcttgaagcc agcatagcag
481agaataaagc atgtttgaag aggacaccta cagaagtttg gagggattct cgaaaccctt
541atgatagaaa aagacaggac aaagctcccg ttgggctaaa gaatgttggc aatacttgtt
601ggtttagtgc tgttattcag tcattattta atcttttgga atttagaaga ttagttctga
661attacaagcc tccatcaaat gctcaagatt taccccgaaa ccaaaaggaa catcggaatt
721tgccttttat gcgtgagctg aggtatctat ttgcacttct tgttggtacc aaaaggaagt
781atgttgatcc atcaagagca gttgaaattc ttaaggatgc tttcaaatca aatgactcac
841agcagcaaga tgtgagtgag tttacacaca aattattaga ttggttagaa gatgccttcc
901aaatgaaagc tgaagaggag acggatgaag agaagccaaa gaaccccatg gtagagttgt
961tctatggcag attcctggct gtgggagtac ttgaaggtaa aaaatttgaa aacactgaaa
1021tgtttggtca gtacccactt caggtcaatg ggttcaaaga tctgcatgag tgcctagaag
1081ctgcaatgat tgaaggagaa attgagtctt tacattcaga gaattcagga aaatcaggcc
1141aagagcattg gtttactgaa ttaccacctg tgttaacatt tgaattgtca agatttgaat
1201ttaatcaggc attgggaaga ccagaaaaaa ttcacaacaa attagaattt ccccaagttt
1261tatatttgga cagatacatg cacagaaaca gagaaataac aagaattaag agggaagaga
1321tcaagagact gaaagattac ctcacggtat tacaacaaag gctagaaaga tatttaagct
1381atggttccgg tcccaaacga ttccccttgg tagatgttct tcagtatgca ttggaatttg
1441cctcaagtaa acctgtttgc acttctcctg ttgacgatat tgacgctagt tccccaccta
1501gtggttccat accatcacag acattaccaa gcacaacaga acaacaggga gccctatctt
1561cagaactgcc aagcacatca ccttcatcag ttgctgccat ttcatcgaga tcagtaatac
1621acaaaccatt tactcagtcc cggatacctc cagatttgcc catgcatccg gcaccaaggc
1681acataacgga ggaagaactt tctgtgctgg aaagttgttt acatcgctgg aggacagaaa
1741tagaaaatga caccagagat ttgcaggaaa gcatatccag aatccatcga acaattgaat
1801taatgtactc tgacaaatct atgatacaag ttccttatcg attacatgcc gttttagttc
1861acgaaggcca agctaatgct gggcactact gggcatatat ttttgatcat cgtgaaagca
1921gatggatgaa gtacaatgat attgctgtga caaaatcatc atgggaagag ctagtgaggg
1981actcttttgg tggttataga aatgccagtg catactgttt aatgtacata aatgataagg
2041cacagttcct aatacaagag gagtttaata aagaaactgg gcagcccctt gttggtatag
2101aaacattacc accggatttg agagattttg ttgaggaaga caaccaacga tttgaaaaag
2161aactagaaga atgggatgca caacttgccc agaaagcttt gcaggaaaag cttttagcgt
2221ctcagaaatt gagagagtca gagacttctg tgacaacagc acaagcagca ggagacccag
2281aatatctaga gcagccatca agaagtgatt tctcaaagca cttgaaagaa gaaactattc
2341aaataattac caaggcatca catgagcatg aagataaaag tcctgaaaca gttttgcagt
2401cggcaattaa gttggaatat gcaaggttgg ttaagttggc ccaagaagac accccaccag
2461aaaccgatta tcgtttacat catgtagtgg tctactttat ccagaaccag gcaccaaaga
2521aaattattga gaaaacatta ctagaacaat ttggagatag aaatttgagt tttgatgaaa
2581ggtgtcacaa cataatgaaa gttgctcaag ccaaactgga aatgataaaa cctgaagaag
2641taaacttgga ggaatatgag gagtggcatc aggattatag gaaattcagg gaaacaacta
2701tgtatctcat aattgggcta gaaaattttc aaagagaaag ttatatagat tccttgctgt
2761tcctcatctg tgcttatcag aaTAACAAAG AACTCTTGTC TAAAGGCTTA TACAGAGGAC
2821ATGATGAAGA ATTGATATCA CATTATAGAA GAGAATGTTT GCTAAAATTA AATGAGCAAG
2881CCGCAGAACT CTTCGAATCT GGAGAGGATC GAGAAGTAAA CAATGGTTTG ATTATCATGA
2941ATGAGTTTAT TGTCCCATTT TTGCCATTAT TACTGGTGGA TGAAATGGAA GAAAAGGATA
3001TACTAGCTGT AGAAGATATG AGAAATCGAT GGTGTTCCTA CCTTGGTCAA GAAATGGAAC
3061CACACCTCCA AGAAAAGCTG ACAGATTTTT TGCCAAAACT GCTTGATTGT TCTATGGAGA
3121TTAAAAGTTT CCATGAGCCA CCGAAGTTAC CTTCATATTC CACGCATGAA CTCTGTGAGC
3181GATTTGCCCG AATCATGTTG TCCCTCAGTC GAACTCCTGC TGATGGAAGA TACCCAACTT
3241TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
3301CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
3361CTTGTGGAAA GGACGAGTTA GACCGGTCGC TGGTCCGGGT ACGCGTTAAG TCgacaatca
3421acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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