Transcript: Human XM_024452239.1

PREDICTED: Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LIF (3976)
Length:
4071
CDS:
265..873

Additional Resources:

NCBI RefSeq record:
XM_024452239.1
NBCI Gene record:
LIF (3976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452239.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000308277 ACCGCATAGTCGTGTACCTTG pLKO_005 581 CDS 100% 4.050 5.670 N LIF n/a
2 TRCN0000058585 CGGCCCTGACACCTCGGGTAA pLKO.1 768 CDS 100% 0.000 0.000 N LIF n/a
3 TRCN0000296724 CAACAACCTGGACAAGCTATG pLKO_005 489 CDS 100% 6.000 4.200 N LIF n/a
4 TRCN0000296662 CTTCTAGCAGGAGGTCTTGAA pLKO_005 867 CDS 100% 4.950 3.465 N LIF n/a
5 TRCN0000058583 CCCATGTCACAACAACCTCAT pLKO.1 378 CDS 100% 4.050 2.835 N LIF n/a
6 TRCN0000058586 TAAGCAGATCATCGCCGTGTT pLKO.1 837 CDS 100% 4.050 2.835 N LIF n/a
7 TRCN0000058587 CCTGTGCCATACGCCACCCAT pLKO.1 362 CDS 100% 0.000 0.000 N LIF n/a
8 TRCN0000290579 CCTGTGCCATACGCCACCCAT pLKO_005 362 CDS 100% 0.000 0.000 N LIF n/a
9 TRCN0000058584 GCAGTGCCAATGCCCTCTTTA pLKO.1 434 CDS 100% 13.200 7.920 N LIF n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452239.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13892 pDONR223 100% 99.8% 4% None 4delA n/a
2 ccsbBroad304_13892 pLX_304 0% 99.8% 4% V5 (not translated due to prior stop codon) 4delA n/a
3 TRCN0000479485 AATTCTTATTAATATCATCTCGCC pLX_317 55% 99.8% 4% V5 (not translated due to prior stop codon) 4delA n/a
Download CSV