Transcript: Mouse XM_030250324.1

PREDICTED: Mus musculus dystrobrevin alpha (Dtna), transcript variant X33, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Dtna (13527)
Length:
1977
CDS:
632..1747

Additional Resources:

NCBI RefSeq record:
XM_030250324.1
NBCI Gene record:
Dtna (13527)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030250324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000108817 GTGATGGTATATGGAAGATAT pLKO.1 1112 CDS 100% 13.200 10.560 N Dtna n/a
2 TRCN0000108819 ACCTGCTAAGAAGCTAACGAA pLKO.1 1513 CDS 100% 3.000 2.400 N Dtna n/a
3 TRCN0000340395 CATGGACAAGTTAAGATATAT pLKO_005 1063 CDS 100% 15.000 10.500 N Dtna n/a
4 TRCN0000340318 TCCAGTGGAGTGATGGTATAT pLKO_005 1103 CDS 100% 13.200 9.240 N Dtna n/a
5 TRCN0000340394 GCACCAAATGAAGGAGTATAC pLKO_005 1480 CDS 100% 10.800 7.560 N Dtna n/a
6 TRCN0000108815 CCTGCTAAGAAGCTAACGAAT pLKO.1 1514 CDS 100% 4.950 3.465 N Dtna n/a
7 TRCN0000108816 GCTGAGATTTGTGCAGAAGAA pLKO.1 751 CDS 100% 4.950 3.465 N Dtna n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030250324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00464 pDONR223 100% 92.8% 99.1% None (many diffs) n/a
2 ccsbBroad304_00464 pLX_304 0% 92.8% 99.1% V5 (many diffs) n/a
3 TRCN0000474295 CCCAAGTAGACGGGTGTAGCACGA pLX_317 37.6% 92.8% 99.1% V5 (many diffs) n/a
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