Transcript: Mouse XM_030250332.1

PREDICTED: Mus musculus potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (Kcnn2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Kcnn2 (140492)
Length:
3423
CDS:
570..3098

Additional Resources:

NCBI RefSeq record:
XM_030250332.1
NBCI Gene record:
Kcnn2 (140492)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030250332.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418185 TATGGTTCTAATCAGCGTTAT pLKO_005 3175 3UTR 100% 10.800 15.120 N KCNN2 n/a
2 TRCN0000069176 GCCGGAGCACAACAATTCTAA pLKO.1 1565 CDS 100% 5.625 7.875 N Kcnn2 n/a
3 TRCN0000434720 AGCTAGAAGAGAATAAGTTAA pLKO_005 3093 CDS 100% 13.200 9.240 N KCNN2 n/a
4 TRCN0000069173 GCACAGGGAATGCACCAATTA pLKO.1 3285 3UTR 100% 13.200 9.240 N Kcnn2 n/a
5 TRCN0000069174 CGGAAATTCTTGCAAGCTATT pLKO.1 2706 CDS 100% 10.800 7.560 N Kcnn2 n/a
6 TRCN0000043843 GCCATGACTTATGAGCGTATT pLKO.1 1971 CDS 100% 10.800 7.560 N KCNN2 n/a
7 TRCN0000069175 GCCAGAGTCATGCTATTACAT pLKO.1 2157 CDS 100% 5.625 3.938 N Kcnn2 n/a
8 TRCN0000069177 GAGACTTTGATTGGTAGCATT pLKO.1 2901 CDS 100% 4.950 3.465 N Kcnn2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030250332.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00901 pDONR223 100% 25.1% 27% None (many diffs) n/a
2 ccsbBroad304_00901 pLX_304 0% 25.1% 27% V5 (many diffs) n/a
3 TRCN0000480327 AAGGTTGAGGTGAGAACAGGAACC pLX_317 51.7% 25.1% 27% V5 (many diffs) n/a
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