Transcript: Mouse XM_030253208.1

PREDICTED: Mus musculus exoribonuclease 3 (Eri3), transcript variant X29, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Eri3 (140546)
Length:
1513
CDS:
374..1000

Additional Resources:

NCBI RefSeq record:
XM_030253208.1
NBCI Gene record:
Eri3 (140546)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030253208.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000178273 GCTCGGTTCTCTTCATATTTA pLKO.1 401 CDS 100% 15.000 21.000 N Eri3 n/a
2 TRCN0000293051 GCTCGGTTCTCTTCATATTTA pLKO_005 401 CDS 100% 15.000 21.000 N Eri3 n/a
3 TRCN0000177778 CCAAACGTCAAGTCAATCTTT pLKO.1 845 CDS 100% 5.625 7.875 N Eri3 n/a
4 TRCN0000293053 CCAAACGTCAAGTCAATCTTT pLKO_005 845 CDS 100% 5.625 7.875 N Eri3 n/a
5 TRCN0000181397 CATGGAAATCGAGTCTACCTT pLKO.1 670 CDS 100% 3.000 4.200 N Eri3 n/a
6 TRCN0000298107 CATGGAAATCGAGTCTACCTT pLKO_005 670 CDS 100% 3.000 4.200 N Eri3 n/a
7 TRCN0000135044 CAAGAACATTGCCAACATCAT pLKO.1 978 CDS 100% 4.950 3.465 N ERI3 n/a
8 TRCN0000178220 GATTACTTCAAGCAGTGGATT pLKO.1 938 CDS 100% 4.950 3.465 N Eri3 n/a
9 TRCN0000293052 GATTACTTCAAGCAGTGGATT pLKO_005 938 CDS 100% 4.950 3.465 N Eri3 n/a
10 TRCN0000135397 GCTTCATCTTCAAGCAGACAT pLKO.1 1019 3UTR 100% 4.950 3.465 N ERI3 n/a
11 TRCN0000134696 GATCCAAACGTCAAGTCAATT pLKO.1 842 CDS 100% 13.200 18.480 N ERI3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030253208.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12532 pDONR223 100% 27.9% 26.2% None (many diffs) n/a
2 ccsbBroad304_12532 pLX_304 0% 27.9% 26.2% V5 (many diffs) n/a
3 TRCN0000466801 TGCCTATACCACAGTATAAGGTTT pLX_317 64.1% 27.9% 26.2% V5 (many diffs) n/a
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