Transcript: Mouse XM_030254586.1

PREDICTED: Mus musculus phosphatidylserine decarboxylase (Pisd), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Pisd (320951)
Length:
2438
CDS:
599..1510

Additional Resources:

NCBI RefSeq record:
XM_030254586.1
NBCI Gene record:
Pisd (320951)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030254586.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115414 CCCTGTCACTATGAATCTACT pLKO.1 90 5UTR 100% 4.950 6.930 N Pisd n/a
2 TRCN0000115415 CAGGTGTCAGAAATTTCCATA pLKO.1 157 5UTR 100% 4.950 3.465 N Pisd n/a
3 TRCN0000417136 CTTACAGAAATTGCCTCTTCA pLKO_005 134 5UTR 100% 4.950 3.465 N Pisd n/a
4 TRCN0000115413 CAGTATGAGAAGTACAGGGAA pLKO.1 258 5UTR 100% 2.640 1.848 N Pisd n/a
5 TRCN0000436785 CCGTATCCACTTTGACCGAGA pLKO_005 1267 CDS 100% 2.160 1.512 N Pisd n/a
6 TRCN0000115411 CCAGTGTCTTCTGAGGACAAT pLKO.1 1999 3UTR 100% 4.950 2.970 N Pisd n/a
7 TRCN0000115412 TCCTACAATGACCTGAGCTTT pLKO.1 1322 CDS 100% 4.950 2.970 N Pisd n/a
8 TRCN0000115421 CGATGGATCAAAGAGCTCTTT pLKO.1 1157 CDS 100% 4.950 2.475 Y Pisd-ps1 n/a
9 TRCN0000115425 CGCCTCAACCAAGTAGAACTT pLKO.1 653 CDS 100% 4.950 2.475 Y Pisd-ps1 n/a
10 TRCN0000078465 CAAGGGCTCCTACAATGACTT pLKO.1 1315 CDS 100% 4.950 3.465 N PISD n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030254586.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02840 pDONR223 100% 69.9% 74.1% None (many diffs) n/a
2 ccsbBroad304_02840 pLX_304 0% 69.9% 74.1% V5 (many diffs) n/a
3 TRCN0000470351 TCTTGCATCGTGTGAACGACTCAT pLX_317 44.3% 69.9% 74.1% V5 (many diffs) n/a
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