Transcript: Human XR_001744577.2

PREDICTED: Homo sapiens transmembrane protein 184A (TMEM184A), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TMEM184A (202915)
Length:
5489
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001744577.2
NBCI Gene record:
TMEM184A (202915)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001744577.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243151 CCTCCAGGCATTTGGCAAATA pLKO_005 750 3UTR 100% 13.200 9.240 N TMEM184A n/a
2 TRCN0000243150 TCAAGTTCCTCACCATCAAAG pLKO_005 2076 3UTR 100% 10.800 7.560 N TMEM184A n/a
3 TRCN0000181214 CAGTATCCTTCTCTGGGAGAT pLKO.1 4899 3UTR 100% 4.050 2.835 N TMEM184A n/a
4 TRCN0000243147 GGCTGTGGGAATCGCTCTTAT pLKO_005 3229 3UTR 100% 13.200 7.920 N TMEM184A n/a
5 TRCN0000243148 TCACCTGCCACCAGATCTATC pLKO_005 366 3UTR 100% 10.800 6.480 N TMEM184A n/a
6 TRCN0000136382 CACCTGTAATTCCAGCACTTT pLKO.1 5378 3UTR 100% 4.950 2.475 Y CENPL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001744577.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13395 pDONR223 100% 22.1% None (many diffs) n/a
2 ccsbBroad304_13395 pLX_304 0% 22.1% V5 (many diffs) n/a
3 TRCN0000473308 CACAAAATAAATTTCGTTCTGTTT pLX_317 24% 22.1% V5 (many diffs) n/a
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