Construct: ORF TRCN0000473308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011048.1_s317c1
- Derived from:
- ccsbBroadEn_13395
- DNA Barcode:
- CACAAAATAAATTTCGTTCTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM184A (202915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 202915 | TMEM184A | transmembrane protein 184A | NM_001097620.2 | 99.5% | 99.2% | 53C>T;1166_1167insCGG;1168A>G |
| 2 | human | 202915 | TMEM184A | transmembrane protein 184A | XM_006715653.1 | 93.2% | 45.2% | (many diffs) |
| 3 | human | 202915 | TMEM184A | transmembrane protein 184A | XM_011515164.1 | 88.7% | 41.5% | (many diffs) |
| 4 | human | 202915 | TMEM184A | transmembrane protein 184A | XM_011515165.2 | 78.4% | 53.1% | (many diffs) |
| 5 | human | 202915 | TMEM184A | transmembrane protein 184A | XM_011515166.2 | 51.7% | 51.4% | 53C>T;645_645delGins598 |
| 6 | human | 202915 | TMEM184A | transmembrane protein 184A | XR_001744577.2 | 22.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag taatgtctca gggatcctgg agacagccgg cgtccccctg gtgtcagtga 121 actggccgca gcccagcccc ccaccggctg tgccagctgg gccgcagatg gaccacatgg 181 ggaacagctc ccagggggcc ccctggctct tcctcacctc cgcactggcc cgaggcgtct 241 cggggatctt cgtgtggact gccctggtgc tcacctgcca ccagatctat ctgcacctgc 301 gctcctacac cgtgccacag gagcaacgtt acatcatccg cctgctcctc atcgtgccca 361 tctacgcctt cgactcctgg ctcagcctcc tcctcctcgg agaccaccag tactacgtct 421 acttcgactc tgtgcgggac tgctacgaag cctttgtcat ttacagcttc ctgagcctgt 481 gtttccagta cctgggaggc gagggcgcca tcatggctga gattcgtgga aagcccatca 541 agtccagctg cttgtacggc acctgctgcc tccggggcat gacctactcc atcgggttcc 601 tgcgcttctg taagcaggcc actctgcagt tctgcctggt gaagcccgtc atggccgtca 661 ccaccatcat cctccaggca tttggcaaat accacgacgg ggacttcaat gtccgcagcg 721 gctacctcta tgtgaccctc atctacaacg cctccgtcag cctcgccctc tacgccctgt 781 tcctcttcta cttcaccacc agggagctcc tgcggccctt ccagcccgtc ctcaagttcc 841 tcaccatcaa agccgtcatc ttcctgtcgt tctggcaagg gctgctgctg gccatcctgg 901 agcggtgcgg ggtcatcccg gaggtggaga ccagcggcgg gaacaagctg ggggctggca 961 cgctggccgc cggctaccag aacttcatca tctgcgtgga gatgctgttc gcctCCGTGG 1021 CCCTGCGTTA TGCCTTCCCC TGCCAGGTGT ACGCAGAGAA GAAGGAGAAT TCACCAGCCC 1081 CCCCGGCACC CATGCAGAGC ATCTCCAGCG GCATCAGGGA GACAGTGAGC CCCCAGGACA 1141 TCGTGCAGGA CGCCATCCAC AACTTCTCCC CCGCCTACCA GCACTACACG CAGCAGGCCA 1201 CGCACGAGGC GCCCAGGCCC GGCACCCACC CCGGCGGCGG CGGCTCCGGC GGGAGCAGGA 1261 AGAGCCGGAG CCTGGAGAAG CGGATGCTGA TCCCCTCGGA GGACCTGTAC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGACACAAAA TAAATTTCGT TCTGTTTACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt