Transcript: Human XR_001744659.2

PREDICTED: Homo sapiens glucuronidase beta (GUSB), transcript variant X7, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GUSB (2990)
Length:
2087
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001744659.2
NBCI Gene record:
GUSB (2990)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001744659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029292 GCCCTGCCTATCTGTATTCAT pLKO.1 932 3UTR 100% 5.625 3.938 N GUSB n/a
2 TRCN0000343994 GCCCTGCCTATCTGTATTCAT pLKO_005 932 3UTR 100% 5.625 3.938 N GUSB n/a
3 TRCN0000029289 CCGAATCACTATCGCCATCAA pLKO.1 543 3UTR 100% 4.950 3.465 N GUSB n/a
4 TRCN0000343993 CCGAATCACTATCGCCATCAA pLKO_005 543 3UTR 100% 4.950 3.465 N GUSB n/a
5 TRCN0000029293 CTGGTATAAGAAGTATCAGAA pLKO.1 1501 3UTR 100% 4.950 3.465 N GUSB n/a
6 TRCN0000343995 CTGGTATAAGAAGTATCAGAA pLKO_005 1501 3UTR 100% 4.950 3.465 N GUSB n/a
7 TRCN0000029290 GCAGAAACGATTGCAGGGTTT pLKO.1 1547 3UTR 100% 4.050 2.835 N GUSB n/a
8 TRCN0000344062 GCAGAAACGATTGCAGGGTTT pLKO_005 1547 3UTR 100% 4.050 2.835 N GUSB n/a
9 TRCN0000029291 GCCAAGTCACAATGTTTGGAA pLKO.1 1838 3UTR 100% 3.000 2.100 N GUSB n/a
10 TRCN0000343996 GCCAAGTCACAATGTTTGGAA pLKO_005 1838 3UTR 100% 3.000 2.100 N GUSB n/a
11 TRCN0000062038 CAAGGGTTACTTTGTCCAGAA pLKO.1 633 3UTR 100% 4.050 2.025 Y GUSBP11 n/a
12 TRCN0000142395 GAACAGCTACTACTCTTGGTA pLKO.1 1423 3UTR 100% 3.000 1.500 Y GUSBP15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001744659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00710 pDONR223 100% 82.6% None (many diffs) n/a
2 ccsbBroad304_00710 pLX_304 0% 82.6% V5 (many diffs) n/a
3 TRCN0000481467 GCCCAGACCTGGCCCATATGTCAG pLX_317 28.2% 82.6% V5 (many diffs) n/a
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