Construct: ORF TRCN0000481467
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010075.1_s317c1
- Derived from:
- ccsbBroadEn_00710
- DNA Barcode:
- GCCCAGACCTGGCCCATATGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUSB (2990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481467
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2990 | GUSB | glucuronidase beta | NM_000181.4 | 100% | 100% | |
| 2 | human | 2990 | GUSB | glucuronidase beta | XM_005250297.4 | 92.1% | 92.1% | 912_913ins153 |
| 3 | human | 2990 | GUSB | glucuronidase beta | XR_927461.3 | 86.7% | 1_45del;1289_1290ins40;1959_2164del | |
| 4 | human | 2990 | GUSB | glucuronidase beta | NR_120531.2 | 84.9% | 1_30del;1421_1422ins85;1899_2114del | |
| 5 | human | 2990 | GUSB | glucuronidase beta | XR_001744659.2 | 82.6% | (many diffs) | |
| 6 | human | 2990 | GUSB | glucuronidase beta | XR_001744660.2 | 79.8% | (many diffs) | |
| 7 | human | 2990 | GUSB | glucuronidase beta | NM_001284290.2 | 77.5% | 77.5% | 472_473ins438 |
| 8 | human | 2990 | GUSB | glucuronidase beta | XR_001744658.2 | 77.5% | (many diffs) | |
| 9 | human | 2990 | GUSB | glucuronidase beta | XR_001744661.2 | 75.9% | (many diffs) | |
| 10 | human | 2990 | GUSB | glucuronidase beta | XM_011516113.1 | 73.1% | 65.5% | 0_1ins329;67_68ins185;209_221del |
| 11 | human | 2990 | GUSB | glucuronidase beta | NM_001293104.2 | 70.6% | 70.3% | (many diffs) |
| 12 | human | 2990 | GUSB | glucuronidase beta | NM_001293105.2 | 66.3% | 62.6% | 0_1ins329;64_65ins328 |
| 13 | human | 2990 | GUSB | glucuronidase beta | XM_017012091.1 | 65.4% | 57.8% | (many diffs) |
| 14 | human | 2990 | GUSB | glucuronidase beta | XM_011516114.2 | 64.4% | 63.7% | 0_1ins685;38_50del |
| 15 | human | 2990 | GUSB | glucuronidase beta | XM_017012092.1 | 62.8% | 62.5% | (many diffs) |
| 16 | human | 2990 | GUSB | glucuronidase beta | XM_017012093.2 | 56.7% | 55.9% | 0_1ins685;38_50del;240_241ins153 |
| 17 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_024054.2 | 28.1% | (many diffs) | |
| 18 | human | 100133050 | LOC100133050 | glucuronidase beta pseudogene | NR_027503.1 | 25.9% | (many diffs) | |
| 19 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_133000.1 | 23.7% | (many diffs) | |
| 20 | human | 102725009 | LOC102725009 | putative inactive beta-gluc... | XM_017030249.2 | 19.9% | 8.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2019
- ORF length:
- 1953
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgggggtcg gcggttgcct gggcggcgct cgggccgttg ttgtggggct 121 gcgcgctggg gctgcagggc gggatgctgt acccccagga gagcccgtcg cgggagtgca 181 aggagctgga cggcctctgg agcttccgcg ccgacttctc tgacaaccga cgccggggct 241 tcgaggagca gtggtaccgg cggccgctgt gggagtcagg ccccaccgtg gacatgccag 301 ttccctccag cttcaatgac atcagccagg actggcgtct gcggcatttt gtcggctggg 361 tgtggtacga acgggaggtg atcctgccgg agcgatggac ccaggacctg cgcacaagag 421 tggtgctgag gattggcagt gcccattcct atgccatcgt gtgggtgaat ggggtcgaca 481 cgctagagca tgaggggggc tacctcccct tcgaggccga catcagcaac ctggtccagg 541 tggggcccct gccctcccgg ctccgaatca ctatcgccat caacaacaca ctcaccccca 601 ccaccctgcc accagggacc atccaatacc tgactgacac ctccaagtat cccaagggtt 661 actttgtcca gaacacatat tttgactttt tcaactacgc tggactgcag cggtctgtac 721 ttctgtacac gacacccacc acctacatcg atgacatcac cgtcaccacc agcgtggagc 781 aagacagtgg gctggtgaat taccagatct ctgtcaaggg cagtaacctg ttcaagttgg 841 aagtgcgtct tttggatgca gaaaacaaag tcgtggcgaa tgggactggg acccagggcc 901 aacttaaggt gccaggtgtc agcctctggt ggccgtacct gatgcacgaa cgccctgcct 961 atctgtattc attggaggtg cagctgactg cacagacgtc actggggcct gtgtctgact 1021 tctacacact ccctgtgggg atccgcactg tggctgtcac caagagccag ttcctcatca 1081 atgggaaacc tttctatttc cacggtgtca acaagcatga ggatgcggac atccgaggga 1141 agggcttcga ctggccgctg ctggtgaagg acttcaacct gcttcgctgg cttggtgcca 1201 acgctttccg taccagccac tacccctatg cagaggaagt gatgcagatg tgtgaccgct 1261 atgggattgt ggtcatcgat gagtgtcccg gcgtgggcct ggcgctgccg cagttcttca 1321 acaacgtttc tctgcatcac cacatgcagg tgatggaaga agtggtgcgt agggacaaga 1381 accaccccgc ggtcgtgatg tggtctgtgg ccaacgagcc tgcgtcccac ctagaatctg 1441 ctggctacta cttgaagatg gtgatcgctc aCACCAAATC CTTGGACCCC TCCCGGCCTG 1501 TGACCTTTGT GAGCAACTCT AACTATGCAG CAGACAAGGG GGCTCCGTAT GTGGATGTGA 1561 TCTGTTTGAA CAGCTACTAC TCTTGGTATC ACGACTACGG GCACCTGGAG TTGATTCAGC 1621 TGCAGCTGGC CACCCAGTTT GAGAACTGGT ATAAGAAGTA TCAGAAGCCC ATTATTCAGA 1681 GCGAGTATGG AGCAGAAACG ATTGCAGGGT TTCACCAGGA TCCACCTCTG ATGTTCACTG 1741 AAGAGTACCA GAAAAGTCTG CTAGAGCAGT ACCATCTGGG TCTGGATCAA AAACGCAGAA 1801 AATACGTGGT TGGAGAGCTC ATTTGGAATT TTGCCGATTT CATGACTGAA CAGTCACCGA 1861 CGAGAGTGCT GGGGAATAAA AAGGGGATCT TCACTCGGCA GAGACAACCA AAAAGTGCAG 1921 CGTTCCTTTT GCGAGAGAGA TACTGGAAGA TTGCCAATGA AACCAGGTAT CCCCACTCAG 1981 TAGCCAAGTC ACAATGTTTG GAAAACAGCC TGTTTACTTG CCCAACTTTC TTGTACAAAG 2041 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2101 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2161 ACGAGCCCAG ACCTGGCCCA TATGTCAGAC GCGTTAAGTC gacaatcaac ctctggatta 2221 caaaatttgt gaaagatt