Transcript: Human XR_001752584.2

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP2K4 (6416)
Length:
2774
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001752584.2
NBCI Gene record:
MAP2K4 (6416)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001752584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039917 GCTCTCCCATGTATGTCGATT pLKO.1 1322 3UTR 100% 4.950 6.930 N MAP2K4 n/a
2 TRCN0000001389 CCTGTGGTCTATTGTCGCTAT pLKO.1 2460 3UTR 100% 4.050 5.670 N MAP2K4 n/a
3 TRCN0000025267 CTTCTGAAACATCCCTTTATT pLKO.1 1225 3UTR 100% 15.000 10.500 N Map2k4 n/a
4 TRCN0000345139 CTTCTGAAACATCCCTTTATT pLKO_005 1225 3UTR 100% 15.000 10.500 N Map2k4 n/a
5 TRCN0000197261 GCATCACGACAAGGATATGAT pLKO.1 888 3UTR 100% 5.625 3.938 N MAP2K4 n/a
6 TRCN0000001390 CTTCTTATGGATTTGGATGTA pLKO.1 486 3UTR 100% 4.950 3.465 N MAP2K4 n/a
7 TRCN0000197078 GATGTAGTAATGCGGAGTAGT pLKO.1 501 3UTR 100% 4.950 3.465 N MAP2K4 n/a
8 TRCN0000001393 GATATGATGTCCGCTCTGATG pLKO.1 901 3UTR 100% 4.050 2.835 N MAP2K4 n/a
9 TRCN0000001392 ACGAGGAGCTTATGGTTCTGT pLKO.1 380 3UTR 100% 3.000 2.100 N MAP2K4 n/a
10 TRCN0000001391 GATGTATGAAGAACGTGCCGT pLKO.1 1248 3UTR 100% 0.660 0.462 N MAP2K4 n/a
11 TRCN0000196996 GACAGCTTGTGGACTCTATTG pLKO.1 808 3UTR 100% 10.800 6.480 N MAP2K4 n/a
12 TRCN0000039914 CCAAAGTGGAATAGTGTATTT pLKO.1 975 3UTR 100% 13.200 6.600 Y MAP2K4 n/a
13 TRCN0000039916 CCCAATCCTACAGGAGTTCAA pLKO.1 240 3UTR 100% 4.950 2.475 Y MAP2K4 n/a
14 TRCN0000197204 GTAAACGCAAAGCACTGAAGT pLKO.1 169 3UTR 100% 4.950 2.475 Y MAP2K4 n/a
15 TRCN0000039915 GTGGGCAAATAATGGCAGTTA pLKO.1 424 3UTR 100% 4.950 2.475 Y MAP2K4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001752584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489440 AAACTTTGGGGGACTTTCACTCAG pLX_317 26.9% 43.1% V5 (not translated due to prior stop codon) 1_53del;1093_1183del;1342_2774del n/a
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