Construct: ORF TRCN0000489440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020206.1_s317c1
- DNA Barcode:
- AAACTTTGGGGGACTTTCACTCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K4 (6416)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6416 | MAP2K4 | mitogen-activated protein k... | NM_003010.4 | 100% | 100% | |
| 2 | human | 6416 | MAP2K4 | mitogen-activated protein k... | NM_001281435.2 | 97.3% | 97.3% | 116_148del |
| 3 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XM_011523976.2 | 74.2% | 72% | (many diffs) |
| 4 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XM_005256753.3 | 70.4% | 70.4% | 0_1ins354 |
| 5 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XM_005256755.2 | 63.4% | 63.4% | 0_1ins438 |
| 6 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XM_011523977.2 | 63.4% | 63.4% | 0_1ins438 |
| 7 | human | 139201 | MAP2K4P1 | mitogen-activated protein k... | NR_029423.1 | 44.5% | (many diffs) | |
| 8 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XR_001752584.2 | 43.1% | 1_53del;1093_1183del;1342_2774del | |
| 9 | human | 6416 | MAP2K4 | mitogen-activated protein k... | XR_934080.2 | 30.4% | (many diffs) | |
| 10 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | NM_009157.5 | 94.7% | 97.7% | (many diffs) |
| 11 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | NM_001316367.1 | 92.1% | 95.1% | (many diffs) |
| 12 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | XM_006533370.3 | 67.5% | 70.1% | (many diffs) |
| 13 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | XM_011249033.2 | 67.5% | 70.1% | (many diffs) |
| 14 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | XM_017314561.1 | 67.5% | 70.1% | (many diffs) |
| 15 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | NM_001316368.1 | 60.7% | 63.1% | (many diffs) |
| 16 | mouse | 26398 | Map2k4 | mitogen-activated protein k... | NM_001316369.1 | 60.7% | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1269
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggct ccgagcccga gcggcggcgg cggctccggg ggcggcagcg 121 gcagcggcac ccccggcccc gtagggtccc cggcgccagg ccacccggcc gtcagcagca 181 tgcagggtaa acgcaaagca ctgaagttga attttgcaaa tccacctttc aaatctacag 241 caaggtttac tctgaatccc aatcctacag gagttcaaaa cccacacata gagagactga 301 gaacacacag cattgagtca tcaggaaaac tgaagatctc ccctgaacaa cactgggatt 361 tcactgcaga ggacttgaaa gaccttggag aaattggacg aggagcttat ggttctgtca 421 acaaaatggt ccacaaacca agtgggcaaa taatggcagt taaaagaatt cggtcaacag 481 tggatgaaaa agaacaaaaa caacttctta tggatttgga tgtagtaatg cggagtagtg 541 attgcccata cattgttcag ttttatggtg cactcttcag agagggtgac tgttggatct 601 gtatggaact catgtctacc tcgtttgata agttttacaa atatgtatat agtgtattag 661 atgatgttat tccagaagaa attttaggca aaatcacttt agcaactgtg aaagcactaa 721 accacttaaa agaaaacttg aaaattattc acagagatat caaaccttcc aatattcttc 781 tggacagaag tggaaatatt aagctctgtg acttcggcat cagtggacag cttgtggact 841 ctattgccaa gacaagagat gctggctgta ggccatacat ggcacctgaa agaatagacc 901 caagcgcatc acgacaagga tatgatgtcc gctctgatgt ctggagtttg ggGATCACAT 961 TGTATGAGTT GGCCACAGGC CGATTTCCTT ATCCAAAGTG GAATAGTGTA TTTGATCAAC 1021 TAACACAAGT CGTGAAAGGA GATCCTCCGC AGCTGAGTAA TTCTGAGGAA AGGGAATTCT 1081 CCCCGAGTTT CATCAACTTT GTCAACTTGT GCCTTACGAA GGATGAATCC AAAAGGCCAA 1141 AGTATAAAGA GCTTCTGAAA CATCCCTTTA TTTTGATGTA TGAAGAACGT GCCGTTGAGG 1201 TCGCATGCTA TGTTTGTAAA ATCCTGGATC AAATGCCAGC TACTCCCAGC TCTCCCATGT 1261 ATGTCGATTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA CTTTGGGGGA CTTTCACTCA 1441 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t