Transcript: Human XR_002956770.1

PREDICTED: Homo sapiens fukutin (FKTN), transcript variant X28, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FKTN (2218)
Length:
7482
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002956770.1
NBCI Gene record:
FKTN (2218)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002956770.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432583 AGTTACAGTTTGGTCGTTATC pLKO_005 825 3UTR 100% 10.800 15.120 N FKTN n/a
2 TRCN0000063763 GCGATCCACTTGGTAGTCTTT pLKO.1 722 3UTR 100% 4.950 3.960 N FKTN n/a
3 TRCN0000435236 TTGTGGAGTCTGAGTTAATAT pLKO_005 2090 3UTR 100% 15.000 10.500 N FKTN n/a
4 TRCN0000423778 CAATGGAATCTGGCCTATTTC pLKO_005 1577 3UTR 100% 13.200 9.240 N FKTN n/a
5 TRCN0000419646 GCCTAGATGAATGAGACAAAT pLKO_005 1839 3UTR 100% 13.200 9.240 N FKTN n/a
6 TRCN0000063767 CCTTTGATACTGGAATTGATT pLKO.1 443 3UTR 100% 5.625 3.938 N FKTN n/a
7 TRCN0000063765 GCTGTTTCAGTTGTACTACTA pLKO.1 277 3UTR 100% 4.950 3.465 N FKTN n/a
8 TRCN0000193660 CAACTATGGTAAGACCTGGAA pLKO.1 1505 3UTR 100% 2.640 1.848 N Fktn n/a
9 TRCN0000350236 CAACTATGGTAAGACCTGGAA pLKO_005 1505 3UTR 100% 2.640 1.848 N Fktn n/a
10 TRCN0000063764 CCTCGAATACATTGAAGCCAA pLKO.1 1487 3UTR 100% 2.640 1.848 N FKTN n/a
11 TRCN0000416772 GGTATCGACAATGCAACATTA pLKO_005 1128 3UTR 100% 13.200 7.920 N FKTN n/a
12 TRCN0000063766 CCAATGCACTTTGTAGAAGAA pLKO.1 917 3UTR 100% 4.950 2.970 N FKTN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002956770.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00547 pDONR223 100% 18.4% None 1_214del;1257_1284del;1626_7482del n/a
2 ccsbBroad304_00547 pLX_304 0% 18.4% V5 1_214del;1257_1284del;1626_7482del n/a
3 TRCN0000477168 TATCTTTCCCAATCGAGACGCCGT pLX_317 23.3% 18.4% V5 1_214del;1257_1284del;1626_7482del n/a
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