Transcript: Human XR_002956976.1

PREDICTED: Homo sapiens WASH complex subunit 2A (WASHC2A), transcript variant X20, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WASHC2A (387680)
Length:
3672
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002956976.1
NBCI Gene record:
WASHC2A (387680)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002956976.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000163697 GCCCATCATAGTGACAATGAA pLKO.1 769 3UTR 100% 5.625 3.375 N WASHC2A n/a
2 TRCN0000370689 GAGTGCCAAGGAGTCATTAAA pLKO_005 2137 3UTR 100% 15.000 7.500 Y WASHC2C n/a
3 TRCN0000365616 GGAAACACCTCAGGCAAATTT pLKO_005 2695 3UTR 100% 15.000 7.500 Y WASHC2C n/a
4 TRCN0000370753 ACGATGAAGAGGATAACTTAT pLKO_005 1067 3UTR 100% 13.200 6.600 Y WASHC2C n/a
5 TRCN0000263954 CCTTGAACCAAAGGATCTATA pLKO_005 582 3UTR 100% 13.200 6.600 Y WASHC2A n/a
6 TRCN0000365545 GAAACAAGTGGACGGACTAAT pLKO_005 252 3UTR 100% 13.200 6.600 Y WASHC2C n/a
7 TRCN0000263953 GAATCCATTCAAGGTAGTAAA pLKO_005 2654 3UTR 100% 13.200 6.600 Y WASHC2A n/a
8 TRCN0000281630 GTCCAATCCACTGCCGATATC pLKO_005 1492 3UTR 100% 10.800 5.400 Y WASHC2A n/a
9 TRCN0000160433 CCATTCAAGGTAGTAAAGAAA pLKO.1 2658 3UTR 100% 5.625 2.813 Y WASHC2A n/a
10 TRCN0000344250 CCATTCAAGGTAGTAAAGAAA pLKO_005 2658 3UTR 100% 5.625 2.813 Y WASHC2A n/a
11 TRCN0000162206 CTCTCTAATACCCAGTTCATT pLKO.1 331 3UTR 100% 5.625 2.813 Y WASHC2A n/a
12 TRCN0000162987 GCTGTGAACTATGGCTTACAA pLKO.1 472 3UTR 100% 5.625 2.813 Y WASHC2A n/a
13 TRCN0000344330 GCTGTGAACTATGGCTTACAA pLKO_005 472 3UTR 100% 5.625 2.813 Y WASHC2A n/a
14 TRCN0000136995 CCTCTGTTAAGGAGGAGTCTT pLKO.1 1232 3UTR 100% 4.950 2.475 Y WASHC2C n/a
15 TRCN0000136398 CGAGAGCAGAAAGAAGTAGAT pLKO.1 430 3UTR 100% 4.950 2.475 Y WASHC2C n/a
16 TRCN0000136217 GCTCTCTAATACCCAGTTCAT pLKO.1 330 3UTR 100% 4.950 2.475 Y WASHC2C n/a
17 TRCN0000137325 GAGGCTGTGAACTATGGCTTA pLKO.1 469 3UTR 100% 4.050 2.025 Y WASHC2C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002956976.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10086 pDONR223 100% 88.1% None (many diffs) n/a
2 ccsbBroad304_10086 pLX_304 0% 88.1% V5 (many diffs) n/a
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