Construct: ORF ccsbBroad304_10086

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010232.1_s304c1, BRDN0000462326
Derived from:
ccsbBroadEn_10086
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WASHC2A (387680)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10086
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 387680 WASHC2A WASH complex subunit 2A NM_001005751.3 99.9% 99.9% 2989C>T
2 human 253725 WASHC2C WASH complex subunit 2C NM_001330074.2 99.2% 98.6% (many diffs)
3 human 387680 WASHC2A WASH complex subunit 2A NM_001291398.1 98.4% 98.3% 2811_2812ins63;2926C>T
4 human 387680 WASHC2A WASH complex subunit 2A XM_005269806.3 98.1% 98% 931_932ins72;2917C>T
5 human 387680 WASHC2A WASH complex subunit 2A XM_005269807.3 97.8% 97.7% 1546_1547ins87;2902C>T
6 human 253725 WASHC2C WASH complex subunit 2C NM_015262.3 97.7% 97% (many diffs)
7 human 253725 WASHC2C WASH complex subunit 2C NM_001367393.1 97.5% 96.9% (many diffs)
8 human 253725 WASHC2C WASH complex subunit 2C NM_001367403.1 97.4% 96.7% (many diffs)
9 human 253725 WASHC2C WASH complex subunit 2C XM_011539568.2 97.1% 96.5% (many diffs)
10 human 387680 WASHC2A WASH complex subunit 2A XM_017016227.1 96.9% 96.8% 2687_2688ins123;2866C>T
11 human 387680 WASHC2A WASH complex subunit 2A XM_017016228.1 96.6% 96.4% 931_932ins72;2739_2740ins63;2854C>T
12 human 387680 WASHC2A WASH complex subunit 2A XM_017016229.1 96.2% 96.1% 1546_1547ins87;2724_2725ins63;2839C>T
13 human 253725 WASHC2C WASH complex subunit 2C XM_017016014.2 96.2% 95.6% (many diffs)
14 human 387680 WASHC2A WASH complex subunit 2A XM_006717831.3 96.1% 96.1% 2138_2139ins153;2836C>T
15 human 253725 WASHC2C WASH complex subunit 2C NM_001367400.1 95.9% 95.2% (many diffs)
16 human 387680 WASHC2A WASH complex subunit 2A XM_017016230.1 95.6% 95.6% 352_353ins174;2815C>T
17 human 253725 WASHC2C WASH complex subunit 2C NM_001367401.1 95.5% 95% (many diffs)
18 human 253725 WASHC2C WASH complex subunit 2C XM_011539569.2 95.4% 94.8% (many diffs)
19 human 253725 WASHC2C WASH complex subunit 2C NM_001367404.1 95.4% 94.7% (many diffs)
20 human 387680 WASHC2A WASH complex subunit 2A NM_001330102.2 95.3% 95.3% 2688_2689ins186;2803C>T
21 human 253725 WASHC2C WASH complex subunit 2C XM_017016017.2 95.3% 94.7% (many diffs)
22 human 253725 WASHC2C WASH complex subunit 2C XM_011539570.3 94.9% 94.3% (many diffs)
23 human 253725 WASHC2C WASH complex subunit 2C NM_001169106.2 94.6% 94.1% (many diffs)
24 human 387680 WASHC2A WASH complex subunit 2A XM_017016231.1 94.6% 94.5% 2138_2139ins153;2658_2659ins63;2773C>T
25 human 387680 WASHC2A WASH complex subunit 2A XM_017016232.1 94.4% 94.3% (many diffs)
26 human 253725 WASHC2C WASH complex subunit 2C NM_001367395.1 94.3% 93.6% (many diffs)
27 human 253725 WASHC2C WASH complex subunit 2C NM_001367408.1 93.9% 93.2% (many diffs)
28 human 253725 WASHC2C WASH complex subunit 2C NM_001367396.1 93.6% 92.9% (many diffs)
29 human 253725 WASHC2C WASH complex subunit 2C NM_001367397.1 93.6% 93% (many diffs)
30 human 253725 WASHC2C WASH complex subunit 2C NM_001367394.1 92.8% 92.2% (many diffs)
31 human 253725 WASHC2C WASH complex subunit 2C NM_001367411.1 92.8% 92.2% (many diffs)
32 human 387680 WASHC2A WASH complex subunit 2A XM_006717832.3 92.8% 92.6% (many diffs)
33 human 253725 WASHC2C WASH complex subunit 2C NM_001169107.2 92.1% 91.4% (many diffs)
34 human 253725 WASHC2C WASH complex subunit 2C NM_001367409.1 91.3% 90.6% (many diffs)
35 human 253725 WASHC2C WASH complex subunit 2C NM_001367410.1 91.2% 90.6% (many diffs)
36 human 253725 WASHC2C WASH complex subunit 2C NM_001367415.1 90.8% 90.2% (many diffs)
37 human 387680 WASHC2A WASH complex subunit 2A XM_011539786.2 90% 90% 2687_2688ins399
38 human 387680 WASHC2A WASH complex subunit 2A XR_001747105.2 89.7% (many diffs)
39 human 253725 WASHC2C WASH complex subunit 2C NM_001367416.1 89.4% 88.8% (many diffs)
40 human 387680 WASHC2A WASH complex subunit 2A XR_002956976.1 88.1% (many diffs)
41 human 253725 WASHC2C WASH complex subunit 2C NM_001367413.1 86.4% 85.8% (many diffs)
42 human 253725 WASHC2C WASH complex subunit 2C NM_001367399.1 86.1% 85.6% (many diffs)
43 human 387680 WASHC2A WASH complex subunit 2A XM_017016233.1 85.5% 85.5% 0_1ins579;2410C>T
44 human 253725 WASHC2C WASH complex subunit 2C NM_001367412.1 84.9% 84.3% (many diffs)
45 human 253725 WASHC2C WASH complex subunit 2C NM_001367402.1 83.4% 82.7% (many diffs)
46 human 253725 WASHC2C WASH complex subunit 2C NM_001367405.1 83.4% 82.7% (many diffs)
47 human 253725 WASHC2C WASH complex subunit 2C NM_001367407.1 83.4% 82.7% (many diffs)
48 human 253725 WASHC2C WASH complex subunit 2C NM_001367414.1 83.4% 82.7% (many diffs)
49 human 253725 WASHC2C WASH complex subunit 2C NM_001367398.1 83.1% 82.4% (many diffs)
50 human 387680 WASHC2A WASH complex subunit 2A XM_017016234.1 81.6% 81.5% (many diffs)
51 human 253725 WASHC2C WASH complex subunit 2C NR_159966.1 77.8% (many diffs)
52 human 253725 WASHC2C WASH complex subunit 2C XR_002956972.1 63.3% (many diffs)
53 human 387680 WASHC2A WASH complex subunit 2A XM_017016235.1 52.9% 50.1% 0_1ins1720;147_148ins170;1099C>T
54 human 387680 WASHC2A WASH complex subunit 2A XM_024447991.1 52.9% 50.1% 0_1ins1720;147_148ins170;1099C>T
55 human 387680 WASHC2A WASH complex subunit 2A XM_024447992.1 52.9% 50.1% 0_1ins1720;147_148ins170;1099C>T
56 human 253725 WASHC2C WASH complex subunit 2C XM_011539572.2 52.7% 49.5% (many diffs)
57 human 253725 WASHC2C WASH complex subunit 2C NM_001367406.1 52.7% 49.7% (many diffs)
58 human 253725 WASHC2C WASH complex subunit 2C XM_017016025.1 52.7% 49.7% (many diffs)
59 human 387680 WASHC2A WASH complex subunit 2A XM_005269810.3 51.8% 49.4% (many diffs)
60 human 253725 WASHC2C WASH complex subunit 2C XM_011539573.3 51.5% 49% (many diffs)
61 human 387680 WASHC2A WASH complex subunit 2A XM_024447993.1 51.4% 48.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4092
ORF length:
4023
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gatgaaccgg acgacccccg accaggagct ggcgccagcg tcggagcccg
121tgtgggagcg gccgtggtcg gtggaggaga tccgcaggag cagccagagc tggtcgctgg
181cggccgacgc gggcctacta cagtttctac aggaattctc acagcaaact atctctagga
241cccatgaaat caagaaacaa gtggacggac taattcggga aaccaaagcc acagattgtc
301gcctgcataa tgtcttcaat gacttcctta tgctctctaa tacccagttc attgagaatc
361gtgtatatga tgaagaagtg gaggagccag tactcaaggc tgaggcagaa aaaacagagc
421aggagaagac acgagagcag aaagaagtag atctcattcc taaagtccag gaggctgtga
481actatggctt acaagtattg gacagtgcct ttgagcaact tgatatcaaa gcaggaaact
541cagactccga ggaagatgat gctaatgggc gggtggaact gatccttgaa ccaaaggatc
601tatacattga tcgtccttta ccatatctca ttgggtcaaa gctgttcatg gaacaagaag
661atgtaggtct tggagagctg tccagtgaag aaggctctgt aggcagtgat cgtggcagta
721ttgtggacac tgaggaagag aaagaagagg aggagtcaga tgaagatttt gcccatcata
781gtgacaatga acaaaaccgg cacaccacac aaatgagtga tgaggaagag gatgatgatg
841gctgtgacct tttcgctgac tctgagaagg aggaggaaga tattgaggac attgaagaaa
901atactagacc taaaagaagc agacctacat cgtttgcaga tgagctggct gcccgcatca
961agggggatgc cgtgggtcga gtggacgagg agccgacaac cttaccctca ggagaagcaa
1021aacctcggaa gacactcaaa gagaagaagg aaaggagaac tccttcagac gatgaagagg
1081ataacttatt cgcacccccc aagctgaccg acgaggactt ctcgccattt ggctctggag
1141gtggcctgtt cagtgggggc aaggggctct ttgatgatga ggacgaggag agtgacctct
1201tcacggaagc cccccaggat cggcaagctg gagcctctgt taaggaggag tcttcatcat
1261ccaaacctgg aaagaaaatc ccagcaggag ctgtttctgt atttttagga gacacggatg
1321tgtttggtgc tgcctccgtt ccatcaatga aggagccaca gaagcctgag cagcccactc
1381caaggaaaag cccctatggt ccccctccca ctggcctctt tgatgatgat gatggtgatg
1441atgatgacga ctttttctcg gcaccccaca gcaaaccttc taaaacaggc aaagtccaat
1501ccactgccga tatctttggt gacgaagaag gagatctgtt caaagaaaaa gccgtagcat
1561cgccagaagc cactgtgagt cagacagatg aaaataaagc aagagcagaa aaaaaggtta
1621ccttatcttc cagcaaaaat ctcaagccct catcagaaac aaagactcaa aaaggcttat
1681tttcagatga ggaggactct gaggatttgt tttcttctca aagtgcgagt aagttaaaag
1741gtgcgtctct gctgcctggc aagctcccca cgttggtttc cctgtttgat gatgaagatg
1801aagaggataa tctttttggg ggtacagctg ctaagaagca gacattgtgt ctacaagctc
1861agagagaaga gaaagcaaaa gcctccgagc tctccaaaaa gaaagcatct gccctgttgt
1921tcagcagtga tgaggaggac cagtggaata ttcctgcttc acagacccac ttagcatctg
1981acagcaggtc taaaggagaa cccagggatt ctgggaccct ccagagccag gaggccaagg
2041ctgtgaaaaa gaccagtctc tttgaggaag acgaagaaga tgatcttttt gccattgcca
2101aggacagcca aaagaagacc cagagagtgt cactcctctt tgaagacgat gttgatagcg
2161gaggctctct gtttggctct cctcccacat ctgttcctcc tgcaacaaag aaaaaagaga
2221ctgtctctga ggcaccacct ttgctgttca gcgatgaaga agagaaggag gcacaacttg
2281gagtgaagtc tgtggataag aaggttgaga gtgccaagga gtcattaaaa tttgggagaa
2341ctgatgtggc tgagtcagaa aaggaaggac ttttgactag atctgctcag gaaacagtca
2401agcattctga tttattttct tcatcatccc catgggacaa aggaaccaag cctagaacca
2461aaactgttct tagcttgttt gatgaggaag aggataaaat ggaagatcaa aacattatcc
2521aggctccaca gaaagaagta ggaaagggcc gcgatcctga tgcccacccc aagagcacag
2581gtgtcttcca ggatgaagag ctgcttttca gccacaagct ccaaaaggac aatgacccag
2641atgttgacct ttttgctggc accaaaaaaa ccaagctgtt agagccaagt gttgggagcc
2701tgtttgggga tgatgaagat gatgatcttt tcagctctgc caagtcccag cctttggtac
2761aagagaaaaa gagagtagtg aaaaaagacc actctgttga ctctttcaaa aaccagaaac
2821atcctgaatc cattcaaggt agtaaagaaa aaggcatatg gaagccggaa acacctcagg
2881actcatcagg tctcgctcca tttaaaacca aagaaccatc cactcggatc gggaagatac
2941aagcaaattt agcgatcaac ccagcggcct tgctgcccac agcggcttcc cagatctctg
3001aagtaaagcc tgttttgcca gaattggctt ttccttcatc tgaacacaga aggagctacg
3061gtctggaaag tgtgcctgtc cttcccggga gtggggaggc cggtgtgagt tttgatcttc
3121cagctcaggc agacacctta cacagtgcaa acaagagccg tgtcaagatg agagggaagc
3181gtagaccgca gacccgtgca gctaggcggc tggctgctca ggagtccagc gagactgagg
3241acatgagcgt ccccagagga cccattgcac agtgggctga tggcgccatt tccccaaatg
3301gccatcggcc acagctcaga gcagccagtg gagaagacag cactgaggag gccctggcag
3361ctgccgctgc accttgggaa ggtggtcctg tgcctggagt ggacagaagc ccctttgcaa
3421agtctctggg tcattccaga ggggaggctg acctttttga ttctggggac atcttttcca
3481cgggcactgg atctcagtct gtggagagaa caaaacccaa ggcaaagata gcagagaatc
3541ctgccaaccc accagtgggt ggtaaagcaa agagccccat gtttcctgct ctaggtgagg
3601ccagcagtga tgatgatctc tttcagtctg ctaaaccaaa accagcaaag aaaacaaatc
3661cctttcctct cctggaagat gaggatgacc tctttacaga tcagaaagtc aagaagaatg
3721agacaaaatc caatagtcag caggatgtca tattaacaac acaagatatt tttgaggatg
3781atatatttgc tacggaagca attaaaccct ctcagaaaac cagagagaag gagaaaacat
3841tggaatctaa tttatttgat gataacattg atatctttgc tgacttaact gtaaaaccaa
3901aagaaaagtc caaaaagaaa gtggaagcca agtctatatt tgatgatgat atggatgaca
3961tcttctcctc tggtatccag gctaagacaa ccaaaccaaa aagccgatct gcacaggccg
4021cacctgaacc aagatttgaa cacaaggtgt ccaacatctt tgatgatccc ctgaatgcct
4081ttggaggcca gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
4141tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
4201caatcaacct ctggatta

Download FASTA (ORF) (Full)