Construct: ORF TRCN0000466721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003368.1_s317c1
- Derived from:
- ccsbBroadEn_11042
- DNA Barcode:
- CGACTGACGATCACGCATCGCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLE (5426)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5426 | POLE | DNA polymerase epsilon, cat... | XM_011534802.3 | 26% | 24.2% | (many diffs) |
| 2 | human | 5426 | POLE | DNA polymerase epsilon, cat... | XM_011534797.3 | 17% | 15.8% | (many diffs) |
| 3 | human | 5426 | POLE | DNA polymerase epsilon, cat... | NM_006231.4 | 16.1% | 16.1% | 1_5748del |
| 4 | human | 5426 | POLE | DNA polymerase epsilon, cat... | XM_011534795.3 | 14.7% | 13.7% | (many diffs) |
| 5 | human | 5426 | POLE | DNA polymerase epsilon, cat... | XR_002957339.1 | 12.7% | 1_6294del;7076_7421del;7751_8692del | |
| 6 | mouse | 18973 | Pole | polymerase (DNA directed), ... | XM_017320715.1 | 14.1% | 14% | (many diffs) |
| 7 | mouse | 18973 | Pole | polymerase (DNA directed), ... | NM_011132.2 | 13.5% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tccatctaac tatggcggaa tcaaaggaaa agtttcatct cgtattcact 121 gtggactgca agactcccag aaagcagggg gagcagagga tgagcaggaa aatgaggacg 181 atgaggagga aagagatggg gaggaggagg aagaggcgga ggaatccaac gtggaggatt 241 tactggaaaa caactggaac attttgcagt ttttgccaca ggcagcctcc tgccagaact 301 acttcctcat gattgtttca gcgtacatcg tggccgtgta ccactgcatg aaggacgggc 361 tgaggcgcag tgctccaggg agcacccccg tgaggaggag gggggccagc cagctctccc 421 aggaggccga gggggcggtc ggagcccttc ccggaatgat caccttctct caggattatg 481 tcgcaaatga gctcactcag agcttcttca ccatcactca gaagattcag aagaaagtca 541 caggctctcg gaactccact gagctctcag agatgtttcc tgtcctcccc ggttcccact 601 tgctgctcaa taaccctgcc ctggagttca tcaaatacgt gtgcaaggtg ctgtccctgg 661 acaccaacat cacaaaccag gtgaataagc tgaaccgaga cctgcttcgc ctggtggatg 721 tcggcgagtt ctccgaggag gcccagttcc gagacccctg ccgctcctac gtgcttcctg 781 aggtcatctg ccgcagctgt aacttctgcc gcgacctgga cctgtgtaaa gactcttcct 841 tctcagagga tggggcggtc ctgcctcagt ggctctgctc caactgtcag gcgcccTACG 901 ACTCCTCTGC CATCGAGATG ACGCTGGTGG AAGTTCTACA GAAGAAGCTG ATGGCCTTCA 961 CCCTGCAGGA CCTGGTCTGC CTGAAGTGCC GCGGGGTGAA GGAGACCAGC ATGCCTGTGT 1021 ACTGCAGCTG CGCGGGAGAC TTCGCCCTCA CCATCCACAC CCAGGTCTTC ATGGAACAGA 1081 TCGGAATATT CCGGAACATT GCCCAGCACT ACGGCATGTC GTACCTCCTG GAGACCCTGG 1141 AGTGGCTGCT GCAGAAGAAC CCACAGCTGG GCCATTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACGACTGACG ATCACGCATC GCGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt