Transcript: Human XR_002958041.1

PREDICTED: Homo sapiens FAM20A golgi associated secretory pathway pseudokinase (FAM20A), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM20A (54757)
Length:
9075
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002958041.1
NBCI Gene record:
FAM20A (54757)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002958041.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000428237 GCCTATCACAAGCTGACTAAA pLKO_005 2299 3UTR 100% 13.200 18.480 N FAM20A n/a
2 TRCN0000172656 GCTCCTCAATGTCATCGACAT pLKO.1 1247 3UTR 100% 4.050 5.670 N FAM20A n/a
3 TRCN0000168142 CATTGACTTTCAGAGACACAA pLKO.1 833 3UTR 100% 4.950 3.465 N FAM20A n/a
4 TRCN0000167128 CTTCTTCTACTTCATTGACTT pLKO.1 821 3UTR 100% 4.950 3.465 N FAM20A n/a
5 TRCN0000168563 GAGGATAGTAAATGTCACCAA pLKO.1 920 3UTR 100% 2.640 1.848 N FAM20A n/a
6 TRCN0000441831 CAGCGATGTGATGCGAGAATC pLKO_005 1667 3UTR 100% 10.800 6.480 N FAM20A n/a
7 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 3562 3UTR 100% 4.950 2.475 Y CFLAR n/a
8 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 3562 3UTR 100% 4.950 2.475 Y C19orf31 n/a
9 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 3560 3UTR 100% 4.950 2.475 Y ERN2 n/a
10 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 3560 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 3560 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002958041.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10241 pDONR223 100% 7.8% None (many diffs) n/a
2 ccsbBroad304_10241 pLX_304 0% 7.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471075 AACATTTGACGGCCCAAGCCAGCC pLX_317 66.7% 7.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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