Construct: ORF TRCN0000471075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018443.1_s317c1
- Derived from:
- ccsbBroadEn_10241
- DNA Barcode:
- AACATTTGACGGCCCAAGCCAGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FAM20A (54757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XR_429905.2 | 60.3% | 1_485del;1226_1227delTG | |
| 2 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XM_011524918.3 | 59.8% | 27.5% | (many diffs) |
| 3 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XM_017024781.2 | 59.3% | 26.3% | (many diffs) |
| 4 | human | 54757 | FAM20A | FAM20A golgi associated sec... | NM_001243746.2 | 57.9% | 25.4% | 304_307delAGAC;695_696ins25;720_1209del |
| 5 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XM_006721959.3 | 57.9% | 25.4% | 304_307delAGAC;695_696ins25;720_1209del |
| 6 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XR_001752543.2 | 48.6% | (many diffs) | |
| 7 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XR_934487.3 | 46.3% | (many diffs) | |
| 8 | human | 54757 | FAM20A | FAM20A golgi associated sec... | NM_017565.4 | 43.3% | 19.2% | (many diffs) |
| 9 | human | 54757 | FAM20A | FAM20A golgi associated sec... | NR_027751.1 | 19.9% | 1_108del;849_3712del | |
| 10 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XR_001752544.2 | 8.2% | 1_485del;789_792delAGAC;1230_8923del | |
| 11 | human | 54757 | FAM20A | FAM20A golgi associated sec... | XR_002958041.1 | 7.8% | (many diffs) | |
| 12 | mouse | 208659 | Fam20a | family with sequence simila... | XM_006532724.3 | 50.5% | 21.9% | (many diffs) |
| 13 | mouse | 208659 | Fam20a | family with sequence simila... | NM_153782.1 | 38.7% | 16.9% | (many diffs) |
| 14 | mouse | 208659 | Fam20a | family with sequence simila... | XR_388388.3 | 23.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cagagagcag atgaacctta cctccctgga ccccccactg cagctccgac 121 tcgaggccag ctgggtccag ttccacctgg gtattaaccg ccatgggctc tactcccggt 181 ccagccctgt tgtcagcaaa cttctgcaag acatgaggca ctttcccacc atcagtgctg 241 attacagtca agatgagaaa gccttgctgg gggcatgtga ctgcacccag attgtgaaac 301 ccagtggggT CCACCTCAAG CTGGTGCTGA GGTTCTCGGA TTTCGGGAAG GCCATGTTCA 361 AACCCATGAG CAGCGAGATG AGGAGACACC AGTGGACTTC TTCTACTTCA TTGACTTTCA 421 GAGACACAAT GCTGAGATCG CAGCTTTCCA TCTGGACAGG ATTCTGGACT TCCGACGGGT 481 GCCGCCAACA GTGGGGAGGA TAGTAAATGT CACCAAGGAA ATCCTAGAGG TCACCAAGAA 541 TGAAATCCTG CAGAGTGTTT TCTTTGTCTC TCCAGCGAGC AACGTGTGCT TCTTCGCCAA 601 GTGTCCATAC ATGTGCAAGA CGGAGTATGC TGTCTGTGGC AACCCACACC TGCTGGAGGG 661 TTCCCTCTCT GCCTTCCTGC CGTCCCTCAA CCTGGCCCCC AGGCTGTCTG TGCCCAACCC 721 CTGGATCCGC TCCTACACAC TGGCAGGAAA AGAGGACCCC AGGACTTCTT CTCCCCCTCA 781 GGTGGGAGGT CAATCCCCTT TACTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 841 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 901 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACATTTGA 961 CGGCCCAAGC CAGCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1021 agatt